1920] 



WALSTER— BARLEY 



ii5 



sugars, and for total sugars after mild hydrolysis. Samples 24 

 and 87 were also analyzed for inorganic phosphorus, using the 

 Powick-Chapin (10) method. F 3 was analyzed for total N, 

 total P, N and P soluble and insoluble in 1 per cent NaOH, phos- 

 phoprotein phosphorus, polysaccharides, and cellulose, etc., by 



TABLE V 



Effect of temperature ox distribution of extractives and insoluble matter 



in barley leaves 



difference. The following list gives the methods employed. 

 The details of the several methods are those recommended by 

 Koch (8) and Mathews (9). 



Total nitrogen Arnold-Gunning method. 



Total phosphorus Neuman-Pemberton method. 



Direct reducing sugars. . .Bertrand volumetric method (glucose calculated from 



Munson-Walker tables in Mathew's Physiological 



Chemistry). 

 Total sugars Bertrand volumetric method applied to the products 



of mild hydrolysis with HC1. 

 Polysaccharides Bertrand volumetric method applied to the products 



of strong hydrolysis with HC1. 

 Phosphoprotein 



phosphorus Determination of the P precipitable by Mg mixture 



in an extract made by 48 hours' digestion with 



1 per cent NaOH at 37-40 C. 



The method for phosphoprotein phosphorus is based upon the 

 discovery by Plimmer and Scott that phosphoproteins can be 

 separated from nucleoproteins through hydrolyzing the former 

 with 1 per cent NaOH, the latter being unattacked by the dilute 



