1921] 



PACK—JUNIPERUS 



47 



using phenolphthalein as the indicator. To show that this increased 

 acid content is real the calculated dry weight of the seed material 

 used is given. 



The fat of the dry seeds is stored as very large globules, con- 

 trary to the statements of Czapek, which divide and become 

 continually smaller as after-ripening goes on. Just preceding 

 germination these fat globules, in the active growing cells, become 

 reduced to microscopic size, although Czapek states that the micro- 

 scopically divided fat of dry seeds collects into globules with early 



TABLE XIII 



Increase of titratable acid in endosperm and embryo 



Condition 



Dry 



Dry 



Dry 



Dry 



After 15 days at 5 C 

 After 30 days at 5 C 

 After 30 days at 5° C 

 After 60 days at 5 C 

 After 60 days at 5 C 

 After 95 days at 5 C 

 After 95 days at 5 C 



Open seeds 



Hypocotyl 2 mm 



Seedlings 



Seedlings 



No. 



80 

 160 

 80 

 80 

 80 

 80 

 80 

 80 

 80 

 80 

 80 

 80 

 80 

 80 

 80 



Dry weight in gm. 



N/50 NaOH in cc. 



Increased acid per 



unit volume of 



water 



0.1865 



growth (7). This dispersion of the fatty material brings clearly 

 into play surface tension, adsorption power, and many other forces 

 resulting from the great increase of specific surface. Such a dis- 

 persion could lead to a more rapid digestion of the fats, thus 

 materially aiding the transformation of fats to carbohydrates and 

 the accumulation of energy for germination. The importance of 

 making the fats more capable of transformation to carbohydrates 

 should not be overlooked. It is also probable that this dispersion 

 reaches a degree of division where it could aid in the translocation 

 of fats as such. Thus highly dispersed fatty material would be 

 carried through the cell walls at points of protoplasmic connection. 



