42 



and within the lymph spaces and blood vessels of the interglandular 

 tissue and the submucosa. 



The character of the ama'hm in sections. — In tolnidine blue and 

 eosin the estosarc stains fairly definitely and the vacuoles of the 

 protoplasm show well. The bacteria also stain, as do the encysted 

 bodies and fragments. The nucleus is very definitely stained and 

 surrounded by a more or less distinct perinuclear space. The 

 nuclear membrane colors a clear deep blue. The nuclear protoplasm 

 appears pink and contains one or several deep blue-black bodies, or 

 perhaps none. There may be several dark-stained thickenings in 

 the nuclear membrane. As a rule, in w'ell-stained sections the 

 amoebffi, where they do not contain much extraneous material such 

 as bacteria and nuclear detritus, are less deeply stained than the 

 cells of the intestinal mucous membrane. The protoplasm of those 

 which are deep in the tissues is less intensely colored than that of 

 the ones in the mucous membrane, and it may be that this phe- 

 nomenon is due to the fact that in the more superficial layers the 

 organisms have taken up more mucous material. That there is 

 some reason for this supposition is shown by the fact that this stain 

 is much less useful in studying the organisms in liver abscesses, 

 wdiere of course there is no mucus. 



Perhaps the most brilliant stain for amoebae in tissues is that of 

 Borrel. This consists of — 



I. Satui'ated aqueous solution magenta red. 

 II. Saturated aqueous solution picric acid. 



Saturated aqueous solution indigo carmin aa. 

 Stain with No. I for twenty minutes and wash. 



Stain with Xo. II for five minutes, wash, and differentiate with alcohol, 

 xylol, and balsam. 



With this, the amoebae are not so readily distinguished by the low 

 powers of the microscope as with the thionin or eosin toluidine-blue 

 stain, but the finer organization is much more easily studied with 

 high powers. Generally with this stain the amoebae are less deeply 

 colored than the surrounding tissues, being a rather pale bluish or 

 purple, or, in well-decolorized specimens, of a greenish hue. The 

 edge of the organisms shows as a fine blue line, which is more 

 distinct about the body of the parasite and less so about the pseudo- 

 podia. The ectoplasm appears as a finely reticular or almost 

 hyaline substance, the fibrillar or granular part of which is stained 

 a very faint blue. The endosare appears as a granular material 



