43 



more deeply stained than the eetosarc, and purplish, bluish, or 

 greenish, according to the degree of decolorization. Within this 

 are spaces which remain uncolored, though in thick sections they 

 have a bluish tint due to the underlying stained material. Bacteria 

 may also be in the endosarc. The rods of which Councilman and 

 Lafleur speak, which are not seen in all cases, occurring in those 

 in which there is more extensive sloughing or diphtheritis, may also 

 be seen. It may be that these are crystalline, derived from the 

 blood, or Charcot-Leyden crystals, which are occasionally found in 

 leucocytes in certain conditions and which Askanazy says are oxy- 

 philic (Munch. Med. ^toch., 1904, LI, 1945). 



The nucleus may be surrounded entirely or in part by a clear 

 perinuclear space. The general color of the nucleus is violet or 

 purplish. The outline is sharp, and, if the section is not too 

 decolorized, should assume a clear crimson tint, in the form of a 

 more or less incomplete ring, or it may be nodulated on its inner 

 surface corresponding to thickenings in the chromatin. There are 

 also occasionally crimson granules within the nucleus and sometimes 

 one perfectly round mass corresponding to the nucleolus. 



Ingested cells, such as red blood corpuscles, leucocytes, etc., may 

 also be seen within the protoplasm in various stages of degenera- 

 tion, the stain depending upon the extent of the process. 



In sections so decolorized that the magenta is all removed, the 

 nucleus appears of a blue color, deeper than the tint of the rest of 

 the cell and with the chromatin material still more deeply stained. 



Heidenhain's iron hematoxylin is as excellent a stain for amoebas 

 as it is for other tissues, although not so brilliant as the magenta- 

 picro-indigo-carmin. In carefully manipulated sections the nucleus 

 of the amoobffi is somewhat more deeply stained than the cystoplasm 

 and ordinarily appears as a dense blue-black ring, in the center of 

 which is the round black nucleolus. The nuclear plasma is usually 

 merely of a blue tint and is divided by a mesh of delicate dark 

 reticulum. There may be other deeply stained chromatic elements 

 or granules within this. The radial striae of the cytoplasm is well 

 shown and the crystalline bodies are, when present, an intense black. 

 The spongioplasm appears as a network of dark lines and the cell 

 boundary is sharply differentiated. Eed cells, when present, stain 

 according to the state of degeneration, those most recently ingested 

 being black; those least so, yellowish. Bacteria, when present, stain 

 sharply and distinctly when not too far degenerated or digested. 



