22 



the tabic, but it has not been thought necessary to include them 

 in this report. By always using the same medium, the same strain 

 of amoebse and symbiotic bacteria, checking every culture and 

 every transplant with controls, using cultures of uniform age 

 (forty-eight hours), cultivating transplants for the same length 

 of time (forty-eight hours), and making the amoeba suspensions 

 of uniform concentration (8 cubic centimeters water to one slant 

 culture forty-eight hours old), the relative results are probably 

 as accurate as possible imder the circumstances : 



Table B. 



Antiseptic. 



Dilution. 



Anti- 

 septic 



ap- 

 plied. 



Growth of 



amcBbse after 



exposure to 



antiseptic. 



Growth of 

 amcpbse on 

 plates inocu- 

 lated with 

 cholera. 



Growth 

 of sym- 

 biotic 

 bacte- 

 ria. 



Growth 



of 

 control. 



Benzoyl acetyl peroxide 



Acid, boric 



Do 



Do 



Do 



Do 



Acid, tannic 



Do 



Acid, succinic peroxide. 



Do- 



Do 



Do 



Argyrol 



Do 



Do 



Do 



Do 



Copper sulphate 



Do 



Do 



Do-i 



Do 



Do 



Eucalyptol (emulsion).. 



Ichthyol 



Oil' of cassia (emulsion) . 

 Do 



Protargol 



Do 



Do 



Potassi um permanganate 



Do 



Do 



Do 



Min. 



1-50 



1-50 



1-50 



1-25 



1-25 



1-100 



1-100 



1-1,000 i 



1-1, 000 



1-1,000 



1-2, 000 



1-100 



1-100 



1-100 



1-500 



1-500 



1-1,000 



1-1,000 



1-1,000 



1-2, 000 



1-2, 000 



1-10, 000 



1-2, 000 



1-500 



1-2, 000 



1-2, 000 



1-500 



1-100 



1-100 



1-2, 000 



1-2, 000 



1-1, 000 



1-4,000 



{■) 



Rich 



do 



Fair 



do 



do 



do 



do 



Rich 



{") 



do 



do 



do 



Rich 



do 



Fair 



do 



do 



Very slight - 



do 



do 



do Fair 



None I Verv slight. 



Slight Fair 



Verv slight do 



do 



None 



Very slight . 



do 



None 



do 



do 



Slight 



None 



Fair 



do 



Slight 



Fair 



Slight 



Very slight. 



do 



do 



None 



do 



Fair 



Very slight . 



Rich 



Very slight . 



do 



Slight 



Very slight . 



Very slight. 



"do 



None 



do 



Fair 



do 



Yes. 

 Yes. 

 Yes. 

 Y'es. 

 Yes. 

 No. 

 No. 

 No. 

 No. 

 No. 

 Yes. 

 No. 

 No. 

 No. 

 No. 

 No. 

 No. 

 No. 

 No. 

 Yes. 

 No. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 No. 

 No. 

 No. 

 Yes. 

 No. 

 Yes. 

 Yes. 



Y'es. 

 Yes. 

 Yes. 

 Y'es. 

 Yes. 

 Y'es. 

 Yes. 

 Yes. 

 Y'es. 

 Yes. 

 Yes. 

 Yes. 

 Y'es. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Ye.s. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Yes. 

 Y'es. 



iNo experiments were performed with this chemical to obtain comparative results 

 on this table, because it had already been proven by other experiments performed 

 in this laboratory that amoebae were always killed by benzoyl acetyl peroxide in 

 the usual dilutions. 



-Two hours. 



"It is not meant to imply either in this table or in the previous one that where 

 a "very slight" growth of amopbie on the sterile plate is recorded and no growth of 

 the symbiotic bacteria, that a pure cultivation of the amoeb;e without bacteria, 

 has been obtained. A very few bacteria may still be present (perhaps adherent 

 to the protozoa) which are sufficient to nourish the amoebae and cause a moderate 

 reproduction of them. In transplanting amoebae another source of error may arise, 

 in regard to their reproduction, where the growth of amoebae is "very slight," 

 unless the number inoculated on the plate has been counted. — R. P. S. * 



