368 



Wuist: Branched prothallia 



Knop's Solution Prantl's Solution 



(o) Ca(N03)2 omitted (a) NH4NO3 omitted 



(6) NaCl and Na3P04 omitted 



(c) NaCl omitted 



(d) Na3P04 omitted 



(e) CaS04 omitted 



After the spores were sown, the cultures were placed before 

 an east window where the conditions of light and temperature 

 were approximately the same for all. 



Description of the branched prothallia 

 In all the cases observed, the germination of the spores, the 

 manner and the rate of growth in the branching prothallia did 

 not differ from that of the unbranched prothallia. Neither were 

 the branched prothallia limited to one kind of culture, but were 

 found in all three kinds. In some of the genera they appeared 

 simultaneously in the three kinds of cultures. 



Fig. 3. Branched prothallia of Onoclea sensibilis L., X 75- 



Onoclea sensibilis L. — The prothallia of this species both on the 

 distilled water and the soil cultures showed a decided tendency to 

 branch. Likewise, the branches showed a great diversity of 

 origin and form, and the beginnings of apical cells or more advanced 



