io8 Organic Phosphorus Compounds of Wheat-Bran [March, 



the similar acid f rom cotton-seed meal, corn and oats, that such dry- 

 ing causes the formation of phosphoric acid. We have found that 

 this is true for our product from wheat-bran. Analytic data: 



0.1919 gm. unheated acid gave 0.0070 gm. MgoPjO,. 



0.2360 gm. acid heated to 105° C. gave 0.0381 gm. MgoPjOj. 



0.2570 gm. unheated acid gave, after heating at 105° C, 0.0210 gm. HjO. 



The unheated acid therefore contains 8.17 percent of water. 

 Stating the results on the dry basis: before heating, 4.00 percent 

 of the total phosphorus was present as phosphoric acid, a part of 

 which may have been produced by the nitric acid of the molybdate 

 Solution. After heating for 2 hr. at 105° C, 16.26 percent of the 

 total phosphorus was present as phosphoric acid. 



Attempt to prepare brucine phytate. Hoping that it might be 

 possible to prepare a crystalline brucine salt of phytic acid, that 

 could be compared with the brucine salt prepared by Anderson from 

 his more complex acid, we undertook to make it by the method 

 used by him, except that to begin with, we did not use the dried 

 acid. This work was done before we effected a Separation of the 

 crude barium salt by water extraction, and the mixture of the water 

 soluble and insoluble materials was therefore used. To 10 gm. of 

 this material, the calculated amount of normal sulfuric acid sol. 

 was added to precipitate the barium. After filtering, the filtrate 

 was concentrated, by boiling in vacuum, to a small bulk. An ex- 

 cess of crystallized brucine was added and, in turn, 150 c.c. of 

 alcohol, 15 c.c. of Chloroform, and ether until a permanent turbid- 

 ity resulted. After standing for two weeks with occasional addi- 

 tion of ether, at a temperature most of the time below freezing, 

 there was not a trace of crystalline deposit, although there was a 

 small amount of gummy material on the bottom of the flask. 



To 3 gm. of the impure barium salt was added the calculated 

 amount of normal sulfuric acid sol., and the liquid, after filtering, 

 evaporated on a water bath, and dried in a water oven for 24 hr. 

 The black residue was dissolved in a small amount of water, alcohol 

 added and the Solution filtered from a small amount of insoluble, 

 carbonaceous matter. Brucine, Chloroform and ether were then 

 added as before and, after standing for i hr. in the laboratory, there 



