1915] Charles J. Robinson and J. Howard Mueller 105 



was, of course, contaminated by some of the dialyzable portion, so 

 that the true barium content must be lower. 



Separation hy extraction with water. This method was sug- 

 gested and used by Anderson** in purifying the phytin derivative 

 from oats. Ten gm. of crude barium preparation were rubbed up 

 in a mortar with about 50 c.c. of water and, after standing for a 

 time, filtered. The residue was extracted twice more in this way 

 with small quantities of water, and finally washed with water, 

 alcohol and ether, and dried. The aqueous extract was shghtly 

 yellow. Addition of alcohol produced, in the first two portions of 

 extract, a rather abundant precipitate; but in the third portion, 

 only a faint turbidity, showing that the extraction was fairly com- 

 plete. The precipitate, after being filtered out and dried, weighed 

 i.i gm. The water-insoluble material was pure white, while the 

 water-soluble f raction was slightly yellow. Analytic data : 



Water-insoluble Fraction. 



Dried for analysis at 100° C. 



0.4972 gm. gave 0.3115 gm. BaSOi. 

 0.3210 gm. gave 0.0650 gm. CO2 and 0.0516 gm. H2O. 

 0.2946 gm. gave 0.1676 gm. MgjPjOj. 

 Found: Ba, 36.87% ; P, 15.86% ; C, 5-52% ; H, 1.80%. 



Calculated: for tri-barium inosite-hexaphosphate, CgHiaOziPcBas — 



Ba, 38.65%; P, 17.44%; C, 6.75%; H, 1.12%. 



Water-soluble Fraction. 



Dried for analysis at 100° C. 



0.4448 gm. gave 0.2560 gm. BaSO«. 

 0.2300 gm. gave 0.0630 gm. CO2 and 0.0410 gm. HjO. 

 Found: Ba, 33-87%; C, 7A7%; H, 2.00%. 



This substance has not been investigated further, 



Preparation of crystalline barium sali. Five gm. of the water- 

 insoluble fraction were dissolved in the smallest possible quantity 

 of dil. hydrochloric acid sol., a sol. of pure barium hydroxid was 

 added to nearly neutralize the free acid, together with 10 gm. of 

 barium chlorid in conc. sol. The mixture was filtered, and alcohol 

 added until a slight permanent precipitate resulted. This precipi- 

 tate was amorphous. After standing over night, it was crystalline 

 er pseudo-crystalline, the material having aggregated in the form 



8 Anderson : Jour. Biol. Chetn., 1914, xvii, p. 160. 



