I04 Organic Phosphorus Compounds of Wheat-Bran [March, 



after resolution in dil. hydrochloric acid sol., was again preclpitated 

 with alcohol. This process was repeated three times more. The 

 material was now free from inorganic phosphates, i. e., it gave no 

 yellow precipitate when warmed with molybdic sol. After wash- 

 ing in alcohol and ether, and drying, the product weighed 57 gm. 

 Dried at 130° C. for analysis, this material turned slightly gray. 

 Analytic data : 



0.2080 gm. gave 0.1235 gm. MgjPoO,. 



0.4736 gm. gave 0.2853 gm. BaSO«. 



1.0058 gm. gave 0.00098 gm. N, by Kjeldahl method. 

 Found: P, 16.55%; Ba, 35-54%; N, 0.098%. 



Calculated: for tri-barium inosite-hexaphosphate, CeHizOjiPeBaa — 



P, 17.44% ; Ba, 38-65%. 



In elementary composition this material approaches the Constitution 

 of a phytin derivative more closely than does Anderson's product, 

 but it is low in both phosphorus and barium. Believing it to be 

 a mixture of tri-barium phytate and some other substance, a means 

 of effecting a Separation was sought. 



Separation by dialysis. One gm. of the material, dried at 

 100° C, was dissolved in dil. hydrochloric acid sol., and placed in 

 a S. & S. No. 579, dialyzing capsule, the latter being put into a 

 beaker of distilled water. After 48 hr., the dialysate and the 

 material remaining in the capsule were precipitated with 3 vol. of 

 alcohol. After filtering out both precipitates, and washing with 

 alcohol and ether, and drying, it was found that the undialyzable 

 material weighed 0.2083 S^- Although precipitation was evidently 

 incomplete, it was piain that some, at least, of the substance was 

 capable of dialysis. Barium was determined in both f ractions : 



0.2083 gm. gave 0.1213 gm. BaSO« (undialyzed fraction). 

 0.3820 gm. gave 0.2408 gm. BaSO« (dialysate). 

 Found: in the dialysate, 37.09 per cent. Ba; in the non-dialyzable fraction, 

 34.27 per cent. Ba. 



From these data it is evident that there are at least two sub- 

 stances in the crude barium-product obtained from the wheat bran, 

 and that the one having the higher percentage of barium is dialyz- 

 able. Since no attempt was made to effect complete Separation, by 

 changing the water in the outer Container, the undialyzed material 



