iQisl Charles J. Robinson and J. Howard Mueller 103 



was found to yield inosite and phosphoric acid on hydrolysis with 

 acid, and hence possibly also on drying. At any rate, we have 

 been unable to obtain a crystallina salt of brucine by using a prep- 

 aration which had not first been heated. After drying about i 

 gm. of the acid at 100° C. for several hours, hovvever, we obtained 

 a good yield of crystals, corresponding in physical properties with, 

 and approximating in composition, Anderson's brucine salt; and 

 also with pure brucine phosphate, prepared and analyzed by us. 



The description of the experimental work is divided into three 

 parts. The first part deals with an investigation of a precipitate 

 obtained by adding copper acetate sol. to an extract of wheat-bran. 

 The second part relates to the material resulting from alcoholic 

 precipitation of bran extract. The third part describes a combina- 

 tion of the two methods. 



Experimental part. i. Precipitate obtained with copper 

 ACETATE. Preparation of the impure harium salt. Five kilos of 

 wheat-bran were extracted over night in 30 1. of 0.2 percent hydro- 

 chloric acid sol., the liquid then strained and pressed out of the 

 residue, and 16 1. more of the 0.2 percent acid sol. added. After 

 stirring at intervals for 2 hr., this was strained out, and the two 

 cxtracts united. After standing for some time, to allow suspended 

 matter to settle out, the supernatant liquid was strained through 

 cotton. To the filtrate was added an excess of conc. sol. of copper 

 acetate, containing some acetic acid. A heavy precipitate was pro- 

 duced. This was allowed to settle over night, the precipitate 

 filtered on a Buchner funnel, and washed two or three times with 

 water. It was then suspended in water, and hydrogen sulfid 

 gas run in for several hours, the mixture being stirred constantly 

 by means of a water motor. The liquid was then filtered from 

 the precipitated copper sulfid. To the filtrate was added a sol. of 

 100 gm. of barium chlorid, and then barium hydroxid sol. to strong 

 alkaline reaction. A heavy precipitate was obtained. This was 

 filtered out, dissolved in dil. hydrochloric acid sol. and filtered from 

 a slight insoluble residue. To the filtrate was again added some 

 barium chlorid and barium hydroxid sol. to alkaline reaction. After 

 filtering and dissolving the precipitate in dilute hydrochloric acid 

 sol, it was precipitated with 3 vol. of alcohol. The precipitate, 



