26 



Serum Diagnosis of Chicken Sarcoma 



[March, 



coma from chemical data for the serum, it does not foUow that 

 analogous results can be obtained for human serum. Our study 

 will be extended in this direction as soon as f urther clinical material 

 is available. By using micro-methods, the amounts of blood to be 

 taken can be very greatly diminished. Very interesting, also, will 

 be the analysis of serum in pregnancy, where differences in other 

 directions may be revealed. 



The method is slightly inconvenient because normal serum must 

 be taken as a control. From the figures obtained it is evident that, 

 by working in pairs (one normal and one tumor serum), both re- 

 sults were relatively either high or low. This was due very likely to 

 the fact that the birds were bled completely, and practically the 

 whole serum was used for analysis. 



TABLE I 



Data pertaining to the alcoholic extraction method on fowls 

 {Blood from the throat: values per loo gm. of serum) 



Experimental. i. The method used was as follows : The blood 

 was taken from pairs (one normal and one tumor animal), and was 

 obtained by cutting the throat of the animal. Both bloods were 

 left for the same length of time in an incubator. The separated 

 serum was weighed and precipitated with 10 times its weight of 

 alcohol. Both precipitates were left in the same desiccator and 

 weighed, and an aliquot part taken for nitrogen and phosphorus 

 estimations. The filtrate was diluted, with the alcoholic washings 

 of the precipitate, to 250 cc. ; 100 cc. were taken for each determi- 



