IQISI 



Casimir Funk 



27 



nation. In estimating small amounts of phosphorus, in a total vol- 

 ume of 50 c.c, by the method described in Hoppe-Seyler-Tierfelder, 

 precipitation frequently failed to occur but could be obtained at 

 greater dilutions. 



These experiments (Table i) show a larger quantity of alco- 

 holic precipitate for normal animals, a corresponding increase in 

 nitrogen, and a slight decrease in phosphorus. In the alcoholic 

 filtrate for tumor animals, there were increases in nitrogen and 

 phosphorus. The experiments were deficient, however, in the fact 

 that, by cutting the throats, the Contents of the crops possibly con- 

 taminated the blood. All the subsequent experiments on fowls 

 were made with blood from the heart, taken by means of a canula, 

 with the animal under anesthesia. 



TABLE 2 



Data pertaining to the alcoholic extraction method on rats 

 {Blood from the throat: values per 100 gm. of serum) 



2. There were eight rats with Ehrlich's sarcoma and six normal 

 ones in this series (Table 2). The method was that described 

 above (i). 



TABLE 3 



Data pertaining to the alcoholic extraction method on fowls 

 {Blood from the heart: values pe'r 100 gm. of serum) 



