54 Physico-Chemical Properties of Vegetable Saps [March, 



CuLTURE A'. American seeds. The young plants were trans- 

 ferred to large pots' of garden soil, which were plunged in the gar- 

 den early in May. Collections were made f rom 24 plants. 



CuLTURE B. This was carried out in precisely the same man- 

 ner as Culture Ä, with the exception that 175 gm. of bone meal 

 was thoroughly mixed with the soil of each pot. Collections were 

 made from 25 plants. 



Culture C. This and the following culture was made from 

 the Haage und Schmidt seed, which was germinated considerably 

 later than the lot that gave rise to the preceding cultures. The 

 young plants were transferred directly from 8 cm. pots into the 

 garden. They were divided into two lots, the first of which (Cul- 

 ture D) was placed in a garden somewhat separated from that in 

 which Cultures A to C were grown. Altogether there were 43 

 plants. 



Culture D. The plants (30) were identical with those of 

 Culture C but grown in the garden separate from that in which Cul- 

 tures A-C were grown. (The gardens were about 200 yards 

 apart. ) 



Culture E. A number (53) of plants from the same sowing 

 as experiments A and B were potted up in 15 cm. pots, in soil which 

 was considerably better than that in the garden. After a few days 

 in the greenhouse to allow the plants to become established, these 

 pots were plunged in the garden adjoining Cultures A and B.* 



The distribution of the collections from which the abnormal 

 fruits were drawn is shown in Table i. 



The entries in this table show that in exp. C and D the materi- 

 als were gathered from well matured vines late in the fall, whereas 

 in the other three series the collections were made at intervals 

 throughout the season. Thus, the entire materials of Culture C 

 were taken for analysis from Sept. 29 to Oct. 3, inclusive. All the 



3 These had an inside diameter of about 29 cm. at the top and 20 cm. at the 

 bottom, with a depth of 30 cm. 



*■ The plan was to give these plants a limited root Space, but, through a mis- 

 understanding on the part of the gardener, the holes in the bottom of the pots 

 were not sufficiently stopped and the soil was drawn up over the tops of the 

 pots, so that roots passed through the bottom and others over the top of the 

 pots. As a result these plants made a more vigorous growth than those in the 

 larger pots. 



