so Intravenous Inject ions of Moiild Sporcs [March, 



made with (^ and ? spores alone in salt Solution. The volume in 

 each tube was 1.5 cc. They were incubated at 2)7 ' C. The spores 

 in the salt Solution, in settling, formed a uniform layer on the bottom 

 of the tube. In the control serum (No. 80), at dilutions greater 

 than I :6oo. the spores also settled uniformly. In dilutions up to 

 I : 1,200, the sera of Nos. 5 and 55 caused a distinct clumping of 

 both J* and ? spores, producing an irregulär roughening of the edges 

 of the spore mass at the bottom of the tubes. A slight but distinct 

 reaction could be observed in the sera of Nos. 5 and 55 with ? spores, 

 and in serum No. 5 with ^ spores at a dilution of i : 2,400. A 

 similar clumping of spores was found in the control serum (No. 80) 

 but only at the tested dilutions of i : 30, i : 150, and i 1300. At 

 dilutions of i : 600, i : 1,200, and i : 2,400 no clumping of spores was 

 observed in the control serum. The reaction seems comparable to 

 the agglutination produced with bacteria. The tests failed to show 

 any specific sexual reaction such as liad been suggested in precipitin 

 tests with the " press-saft " from the mycelium of this same mold 



(5)- 



It was not possible to carry on the work with the sera further at 

 this time. They were inactivated at 56" C. for one half hour on 

 Aug. 26, and later taken to Storrs, Conn., where they were pre- 

 served in an icebox. Pressure of other work prevented a repetition 

 of the tests until Feb. 15, 19 14. Despite the age of the sera the 

 results were in general confirmatory of the previous tests. Fresh 

 serum from another rabbit (No. 30) was used as a control. The 

 spore mixture was estimated to contain somewhat over twice as many 

 spores as were previously employed. Their greater number may 

 in a measure account for the fact that in the higher dilutions of the 

 control serum the spores, instead of forming a uniform layer on the 

 bottom of the test tubes as had been the case in the control serum six 

 months before, now settled together into a dense dot. Plate 2 

 shows (^ spores in tubes of serum at a i : 320 dilution, together with 

 spores in salt Solution alone. The tubes were slightly slanted after 

 the spores were added and the slant reversed to show the character 

 of the settled spores in the photograph. The roughening of the 

 edges of the spore masses in sera Nos. 5 and 55 is a characteristic 

 appearance. The spores in No. 55 were slightly disturbed in 

 handling. 



