48 



Intravenous Injections of Mould Spores 



[March, 



rabbits were all females of about the same age. At the time of the 

 final injections No. 5 weighed 2130 gm. ; No. 6, 2160 gm.; No. 55, 

 1900 gm.; No. 66, 1730 gm. 



The number of spores were estimated by counts with a hema- 

 cytometer. Loops of blood were taken from these rabbits at half 

 hourly, hourly, and eventually at less freqnent intervals. The 

 blood, taken from a slight prick in an ear vein, was at once mixed 

 with sterile water in small vials, and as soon as possible made into 

 Separation cultures in Lindner roll-tubes (4 ) . The last injection was 

 made in the left ear and the blood to be tested was always taken 

 from the right ear. 



TABLE 2 



Figures indicate niimbcr of viahlc spores in loop taken from tubes of scrum or 



Salt Solution at intervals indicated 



Table i shows the number of viable spores in the loops taken, as 

 indicated by the number of colonies developing in these roll-tube 

 Separation cultures. The quantities of blood taken up in the loops 

 unavoidably varied, and thus no doubt, influenced considerably the 

 number of spores found at each test. The number of spores in- 

 jeeted into the rabbits could not be exactly the same and they may 

 have been for some time unequally distributed in the blood. Such 

 experimental errors may account for a considerable Variation in the 

 number of spores found in the individual loops but can hardly ex- 

 plain the relatively large total count for rabbit No. 5. An indi- 

 vidual peculiarity of this animal may be responsible for the larger 



