ipis] Charles J. Robinson and J. Howard Mueller 115 



0.1974 gm. gave 0.1211 gm. BaSOi. 

 0.2068 gm. gave 0.1116 gm. MgoPsOj. 

 0.3655 gm. gave 0.0794 gm. CO; and 0.0629 gm. H2O. 

 Fotind: Ba, 36.10%; P, i5-53%; C, 5-93%; H. 1.93%- 



'Attempt to crystallize the water-insoluble fraction. All the 

 water-insoluble material was dissolved in 0.2 percent hydrochloric 

 acid sol., and alcohol added until a fairly heavy amorphous precip- 

 itate was obtained. After Standing several days, this precipitate had 

 in part crystallized, but there was considerable amorphous matter 

 mixed with the crystals. The form of the crystals was identical 

 with that of the crystals obtained before in pure form. Upon 

 Standing for several days, complete crystallization failed to take 

 place, and the mixture of crystals and amorphous matter was filtered 

 out and dried for analysis at 105° C, in vacuum, over phosphorus 

 pentoxid. Analytic data : 



0.1569 gm. gave 0.0942 gm. BaSO,. 

 0.1525 gm. gave 0.0889 gm. MgoPoOi. 

 0.2455 gm. gave 0.0570 gm. CO, and 0.0391 gm. H^O. 

 Found: Ba, 35-35%; P, 16.87% ; Q 6.33%; H, 1.78%. 



None of these substances bears any resemblance to the material 

 obtained by us as described in the second part of this paper. No 

 satisfactory reason suggests itself for this fact, although there were 

 three differences in the methods of preparation: first, a larger 

 amount of alcohol was used, securing a more complete precipitation ; 

 second, tannic acid was not added to the original extract ; and third, 

 the extraction and precipitation of the Compound were completed in 

 three days, whereas two weeks were consumed for the first prepara- 

 tion. The latter fact could make a difference only if the material 

 tends to decompose. Believing that these differences of procedure 

 are not adequate to explain the disparities in composition, we leave 

 the question open for f urther investigation, with the Suggestion that 

 there may possibly be differences in wheat-brans, one of the Com- 

 pounds being formed first and converted gradually, by the metabo- 

 lism of the plant, into the other. 



Conclusions, A large part of the organic phosphorus of wheat- 

 bran exists as phytin, similar to that from many other sources. A 

 crystalline tri-barium salt may readily be prepared from it. This 

 material is most readily obtained by the copper acetate method. 



