1915] Mark J. Gottlieb and Seymour Oppenheimer 131 



This we have accomplished in one of our cases. From G. G., 

 a patient who received forty-five injections of ragweed extract, 

 we took about two ounces of blood from a vein. After the proper 

 precaution of a Wassermann reaction, we injected subcutaneously 

 8 c.c. of the serum into a patient thirteen years of age, during a 

 violent attack of hay fever. Before the expiration of thirty-six 

 hours all Symptoms of hay fever disappeared from this little patient 

 and no signs of the disease returned during the entire season. 



3. By injecting very small amounts of pollen extract at inter- 

 vals of ten days or less, so that only minute quantities of anaphyla- 

 toxin are formed and the patient's tolerance is raised. 



4. By injecting very small doses of anaphylatoxin made in vitro 

 to produce the same results as in method 3. 



Practical considerations. It has been our object to im- 

 munize our patients by injecting gradually increased doses of pollen 

 extract to produce tolerance to the anaphylatoxin formed in the 

 body, Beginning with 1-5 units of pollen extract, the dose was 

 gradually increased until a local reaction appeared at the site of the 

 injection. This dose was then continued until the patient showed 

 no more reaction. Then the dose was gradually increased as before. 



One Unit of pollen toxin was the amount of antigen dissolved in 

 I c.c. of extract at a dilution of i : 20,000,000. 



Method of preparing Vaccine. Flowers were dried, stripped 

 from their stems, and crushed by hand. This material was then 

 enclosed in muslin bags of suitable size and thoroughly shaken in 

 a large bottle. This bottle contained a cheese-cloth-covered, in- 

 verted, funnel connected by rubber tubing to a suction flask with the 

 outlet in the latter protected by a silk filter. As the fine powder 

 was shaken from the bag into the bottle, the air current carried it 

 into the funnel and thence into the flask, where the silk filter helped 

 to prevent loss of pollen grains. This method was partly success- 

 ful with ragweed but of no use with golden-rod. 



It was thought likely that golden-rod flowers needed a greater 

 pulverization to free the pollen from the anthers. Flowers were 

 accordingly put into a ball-mill and a fine dust was obtained. Sedi- 

 mentation experiments were then undertaken with this powder to 

 determine what concentration of alcohol in water, and alcohol 



