I9I51 Edgar G. Miller, Jr. 205 



Chromates may evidently be used in many relations as a quali- 

 tative protein reagent instead of potassium ferrocyanid, and they 

 prove more advantageous under certain conditions because excess 

 is easily eliminated from Solution. They should be useful as 

 quantitative precipitants of various proteins. We have used the 

 method successfully to remove protein from Solutions prior to the 

 Isolation of such associated substances as ovomucoid and glycogen. 

 It is our Intention to continue this study. 



148. Active immunization to hay fever. Mark J. Gottlieb 

 and Seymour Oppenheimer. Published in this issue : Biochem. 

 Bull., 1915, iv, p 127. 



149. Nitrogen metabolism in experimental uranium ne- 

 phritis. Herman O. Mosenthal. Nitrogen metabolism in ex- 

 perimental uranium nephritis bears certain resemblances to that in 

 clinical nephritis. Urinary nitrogen may, in both of these condi- 

 tions, be increased or diminished from the outset of the kidney in- 

 volvement. It appears certain that retention of nitrogen is due to 

 insufficient excretory powers of the kidney. Increased excretion 

 is, in the experimental type of the disease at least, not due to pre- 

 vious nitrogen retention but to increased protein catabolism. This 

 is proved by the fact that in dogs poisoned with uranium, urinary 

 nitrogen is present in excess as compared with the intake, while at 

 the same time non-protein nitrogen of the blood is markedly in- 

 ■creased in amount. 



A study of other phases of nitrogenous metabolism, when the 

 blood and urine present the phenomena just stated, shows that the 

 fecal nitrogen is unchanged in amount, and the quantity of nitrogen 

 in the succus entericus, as determined by the Thiery-fistula method, 

 tends to diminish. During the nephritic period, blood-pressure 

 rises and remains above normal after all signs of renal disease dis- 

 appear. Other urinary constituents — chlorids, sulfur and phos- 

 phates — fail to parallel the nitrogen in excretion. Even individual 

 urinary nitrogenous fractions, e. g., uric acid, may be retained 

 while total nitrogen is increased. 



These facts indicate that uranium nephritis implicates the body 

 as a whole and not the kidneys only. Furthermore, the various 

 functions of the body do not necessarily Supplement each other by 



