1915] Edgar G. Miller, Jr. 209 



by reading Bunting's chemical comedies, the more he is likely to kill himself— 

 kill himself laughing! 



Bunting's Suggestion that Gies' discussion of his (Bunting's) paper " reveals 

 plainly the fact that he (Gies) has not done the work which he claims to have 

 performed upon this method " leads us to propose that Gies be promptly inves- 

 tigated. It is suggested that the junior author be called as the first witness and 

 that Dr. A. P. Lothrop, who tested the validity of some of our conclusions, be 

 called as the second.] 



151. Absorption of unaltered protein through the gastro- 

 enteric tract in infants. O. M. Schloss. The infants tested 

 were given the whites of one to two eggs. The urine was collected 

 for 6 hr. and used for precipitin tests. Urine, or protein precip- 

 itated from urine by Saturation with ammonium sulfate, was in- 

 jected into the peritoneal cavity of guinea-pigs and, 21 days later, 

 similar injection of egg- white was made. The precipitin reaction 

 was positive in very few normal infants, but was positive in a large 

 percentage of those suffering from gastro-enteric disorders or mal- 

 nutrition. In many instances the positive precipitin reaction was 

 coincident with albuminuria (heat and acetic acid test) but, in a 

 number of urines giving positive precipitin tests, no albumin was 

 demonstrable. The anaphylactic reaction was positive in practi- 

 cally all cases with both albuminuria and a positive precipitin re- 

 action, but was uniformly negative when no albuminuria was 

 present. The results indicate that unaltered, or but slightly altered, 

 protein can be absorbed from the gastro-enteric tract of infants 

 suffering from nutritional disorders. 



Tests were also made for protease in the blood-serum of infants. 

 The dialytic technic of Abderhalden was used, and proteins from 

 tgg and milk were employed. Proteases were present in a few 

 normal infants and in a large percentage of those suffering from 

 nutritional disorders. 



One of the characteristic anaphylactic reactions in the guinea- 

 pig is a marked rise in the eosinophile blood-cells. It seemed of 

 interest to determine whether, in the continued feeding of foreign 

 protein, sufficient would be absorbed to cause such a reaction. 

 Guinea-pigs were fed approximately 5 gm. of powdered egg-white 

 a day. Four of the six animals thus fed showed marked increases 

 in eosinophile blood-cells accompanied by leukocytosis. Control 

 animals showed no such blood changes. 



