I9I5] Edgar G. Miller, Jr. 213 



new growths on agar-agar. We may therefore conclude that the 

 living cell is not essential f or light production ; and, f urther, that the 

 photogen is not a fat, or a lecithin, or any ether-soluble substance. 



Luminous bacteria require free oxygen in order to phosphoresce. 

 If the bacteria could be broken up in an oxygen- free medium, any 

 photogenic substance should dissolve in the medium and phosphor- 

 esce when oxygen is again added. The bacteria were broken up 

 (cytolyzed) by adding (a) toluene to a bacterial emulsion in oxygen- 

 free sea- water and (b) oxygen- free distilled water to a dense mass of 

 bacteria in a Space devoid of oxygen. After 10-15 ^ni"-» oxygen 

 was admitted but in neither case did phosphorescence appear. We 

 may therefore conclude that the photogen is unstable and breaks 

 up without the production of light in water free from oxygen. Of 

 course the photogen would rapidly burn up if any free oxygen were 

 present. 



Exactly similar results were obtained with the dry powdered 

 luminous organs of the fire-fly. (See Jour. Amer. Chem. Soc, 

 1915, xxxvii, p. 396.) 



158. Metabolism studies of two cases of hemophilia. Al- 

 fred F. Hess and Max Kahn. (Chem. Lab., Beth Israel Hosp., 

 N. Y. City.) The intake and Output of nitrogen, sulfur, phos- 

 phorus, chlorin, calcium, magnesium and fat were studied in two 

 cases of hemophilia. It was found that in one of these cases (B. A.) 

 there was a minus calcium balance which could be changed to a plus 

 balance by administering calcium chlorid per os daily. It was 

 found that this case also had a diminished calcium content in the 

 blood. The other case (J.) was normal, so far as was shown by 

 the metabolism experiments or calcium "content of the blood. In 

 the first case, we used a sol. of calcium chlorid, in salin, of such 

 strength that the addition of i drop of it to 10 drops of blood ex- 

 actly supplied the amount of calcium that the blood seemed to lack. 

 It was found that the coagulation time of blood so treated was 

 reduced from about 45 to 50 min., to 10 or 12 min. 



159. Calcium metabolism in normal and diabetic individ- 

 uals. Max Kahn and Jacob Hoffmann. (Chem. Lab., Beth 

 Israel Hosp., N. Y. City.) Diabetic patients who excreted sugar 

 in the urine showed a distinct daily calcium loss. Administration 



