1915] Edgar G. Miller, Jr. 215 



Among the Streptococci such a correlation, if it exists, would be 

 especially significant in that it would help to differentiate the vari- 

 ous members of a genus that has puzzled many investigators. 



The agglutination, fermentation and hemolytic properties of 

 sixty strains derived from various pathological conditions were 

 studied, using four agglutinating sera having a titre of 800-1000; 

 and six carbohydrates and other fermentable substances as f ollows : 

 Disaccharides— /ac^o.?^_, sucrose; trisaccharide — raffinose; alcohol 

 — mannite; glucoside — salicin; Polysaccharide — inulin. 



Only twenty-seven of the strains were agglutinated by the sera 

 used. A definite correlation was, however, obtained between the 

 agglutinative and fermentative characters. The serum produced 

 by a strain of one fermentative group (the group that fermented 

 salicin, for instance) agglutinated only cultures of its particular 

 division and failed to agglutinate members of any of the other 

 groups. No such correlation was obtained with the hemolytic 

 property, members of one hemolytic group being agglutinated by 

 the sera produced by strains from another hemolytic group. 



The results indicate that a Separation of the Streptococci ob- 

 tained from various pathological conditions, into three fermentative 

 types, would coincide most closely with their natural relationship. 

 The groups suggested are : 



(A). Salicin fermenters only, generally hemolytic. — Str. 

 pyogenes. 



(B). Raffinose fermenters; salicin usually fermented; man- 

 nite always negative; generally produces a green colony on blood 

 agar. — Str. salivarius. 



(C), Mannite fermenters; generally ferment salicin; rarely 

 ferment raffinose; variable in their reaction to blood. — Str. fecalis. 



163. The reducing power of anerobes. Victor E. Levine. 

 (Dep't of Public Health, Amer. Museum of Natural Hist., N. Y. 

 City.) It is a well established fact that anerobes reduce organic 

 dyes, such as methylene blue (Cahen, Smith, Kitasato and Weyl). 

 No difference on the ground of reduction may be claimed between 

 aerobes and anerobes. Klett,^^ however, using sodium selenite as 

 an indicator, found that the anerobes he examined lacked the power 



1* Klett : Zeit. f. Hyg., 1900, xxxiii, pp. 135, 137. 



