1915] Edgar G. Miller, Jr. 197 



in dilutions above i : 100,000. Dilutions of i : 25,000 and i : 10,000 

 gave the best results. Reduction occurred only on the surface and the 

 growth was colored brick red, due to the deposition of selenium par- 

 ticles, which, under the microscope, appeared to be in the cell, indicat- 

 ing that the reduction was intracellular. After a few days the color 

 began to fade and a characteristic oder of volatile selenium was 

 produced. None of the organisms was inhibited in growth by any 

 of the dilutions used. There were no sharp distinctions in types of 

 growth of the various strains, though the diphtheria bacilli gave 

 characteristic discreet colonies, which differed markedly from those 

 of the pseudo-diphtheria. This was especially evident in the higher 

 concentrations after 12 to 18 hr. growth. It is uncertain whether 

 the difference is sufficiently constant to be of diagnostic value. The 

 experiments are in progress. 



139. On the presence of oleic and other unsaturated acids 

 in the gastric Contents. Max Kahn and J. Subkis. (Chem. 

 Lab., Beth Israel Hosp., N. Y. City.) After administering to the 

 patient a piece of bread and a glass of water, and withdrawing the 

 gastric contents, the authors determined, by Hübl's method, the 

 amount of iodin absorbed by the filtrate. Gastric contents of low 

 acidity had a small iodin number; of high acidity, a relatively large 

 iodin number. These results contradict Graf, who stated that the 

 gastric juice of patients suffering from Carcinoma of the stomach 

 contains an appreciable amount of oleic acid. 



140. A Volumetrie determination of Sulfates in urine. 

 Alwyn Knauer and Benjamin Horowitz. (Physiol. Lab., 

 Fordham Univ. Med. Seh., N. Y. City.) Solutions required: (i) 

 Barium chlorid, i cc. = 0.005 S^- of suKuric acid; (2) potassium 

 Sulfate, I cc. = I cc. of sol. (i). 



I. Total {inorganic and ethereal) Sulfates: 50 cc. of urine and 

 5 cc. of hydrochloric acid sol. (sp. gr., 1.2) are poured into an Er- 

 lenmeyer flask, and the mixture boiled for about 5 min. Barium 

 chlorid sol., i, usually 10-15 cc, is then added and the mixture 

 boiled 3-4 min. longer. Three small test tubes, of uniform bore and 

 perfectly clean, are now placed side by side in a test tube rack, and 

 labelled A, B and C. Small portions (not more than i cc.) of the 

 sol. are filtered into each, using a very small funnel and a very small 

 iilter paper for the purpose. A is kept as a control. To 5 3 drops 



