368 Biochemistry of Cod-Liver Oil [June-September 



clpitate with mercuric chlorid. Before using this reagent, others 

 were tried, but without success. The decomposed phosphotung- 

 state precipitate had a very pronounced smell of nitrogenous bases. 

 The final sol. of free bases was precipitated with mercuric chlorid in 

 alcoholic sol. Both the precipitate and the filtrate gave crystalline 

 Chlorids after elimination of mercury and evaporation. 



Treatment of the phosphotimgstic acid filtrate. The filtrate was 

 freed from phosphotungstic and sulfuric acids by means of neutral 

 lead acetate. The filtrate, freed from lead with hydrogen sulfid, 

 was conc. in vacuo. It was thought likely that it contained a large 

 amount of amino-acids derived from liver tissue. The whole sol. 

 contained 4.5 gm. of nitrogen and only 0.24 gm. of amino-nitrogen, 

 as determined by Van Slyke's method. The residue, after evapora- 

 tion, was entirely soluble in alcohol with the exception of a small 

 quantity of inorganic material. The liquid was freed from chlorid 

 with silver acetate, and the silver removed with hydrogen sulfid. 

 The residue, when dissolved in alcohol and slowly evaporated, gave 

 a crystalline substance. This will be investigated in the near future. 



Treatment of the fatty residue obtained from the evaporated 

 alcohoUc-aqiieous extracts. This oil was hydrolyzed with 5 percent 

 sulfuric acid sol. for 2 hr. The filtered liquid, which smelt like 

 herring, was precipitated with phosphotungstic acid; 19.5 gm. of 

 dry precipitate were obtained. 



Treatment of oil extracted with dilute alcohol. The extracted 

 oil was hydrolyzed for 2 hr. with 5 percent sulfuric acid sol. The 

 acid extracts were precipitated with phosphotungstic acid; 186 gm. 

 of precipitate were obtained. 



2. About 25 k. of the same supply of dark oil were extracted in 

 portions of 2 k. each with 2 1. of 10 percent sulfuric acid sol. for 2 

 hr. on a shaking machine, and then lef t over night. The extracts were 

 isolated in a separatory funnel and precipitated with phosphotung- 

 stic acid. The resulting precipitate (dry), which weighed 877 gm., 

 was extracted with acetone and 57.2 gm. of insoluble fraction ob- 

 tained. A second extraction of the oil, as above, yielded 240 gm. 

 of phosphotungstate but only 4 gm. of the acetone-insoluble fraction. 



Treatment of the acetione-insoluble fraction. The 61.2 gm. of 

 material insoluble in acetone, obtained by the above mentioned treat- 



