191 5] Casimir Funk 34i 



The Salt mixture contained lo gm. of calcium phosphate, 40 gm. 

 of potassium bi-phosphate, 20 gm. of sodium chlorid, 15 gm. of 

 sodium citrate, 8 gm. of magnesium citrate, and 8 gm. of calcium 

 lactate. This diet was inadequate for mice; they grew less rapidly 

 than on a diet of bread and milk. The Substitution of vitellin for 

 the nucleoprotein did not yield better results. No pathological alter- 

 ations were seen in these animals and, on changing this diet to a 

 normal one, quick recoveries followed. The second diet gave good 

 results in old mice, but in young ones no growth was observed. The 

 Substitution of a part of the vitellin in the second diet by chicken 

 protein, or edestin, failed to affect growth. The same was true of 

 meat-extract and meat-powder. On the other band, addition of 

 malt-extract or nucleoprotein from liver stimulated growth. Egg- 

 yolk fat had an unfavorable effect on adult mice. In young mice 

 egg-yolk prolonged life but did not stimulate growth. Very good 

 results were also obtained when the food was mixed with yeast 

 and baked ; lecithin was without effect. 



Hart and McCollum (119) performed some experiments on 

 swine. No growth was observed either on wheat alone, or on corn 

 meal and gluten feed. Growth occurred, however, when to the 

 above mentioned food, potassium phosphate, potassium citrate and 

 calcium lactate were added. Under these conditions the growth on 

 corn meal was more successful. 



Wheeler (120), in Mendel's laboratory, extended to mice, the rat 

 studies of Osborne and Mendel, and found that mice are also well 

 adapted for experiments on growth. Wheeler's work was under- 

 taken at a time when Osborne and Mendel believed in a special 

 relation between milk-salts and growth, but the results indicated the 

 necessity of adding organic milk food, even in larger relative quan- 

 tity than for rats, to induce complete growth, in which case mice 

 grew more rapidly than rats. Osborne and Mendel (121) have 

 amended their statement regarding the beneficial effect of protein- 

 free milk, and of artificial protein-milk, on the growth of rats. 



Hart and McCollum (122), in continuation of their studies on 

 growth in swine, concluded that even after an addition of salts to 

 corn- or wheat-feed only partial growth can be obtained, the pigs 

 showing signs of paralysis with ultimate decline. 



