had caused au injury, tlius enabling tlie fungus to develop 

 there; but this boll was not affected with the disease in 

 question. 



Hence it is demonstrated that this specific bacterium was 

 and is the cause of the disease in question. 



From one of the original bolls some diseased tissue includ- 

 ing seed was hardened in increasing strengths of alcohol, 

 infiltrated with paraffine in the usual manner, cut into sec- 

 tions which were fastened to the slide by clove-oil-collodion, 

 stained with gentian violet or with car])ofuchsin, and 

 mounted in balsam. On examination with a high power 

 (1-24 inch Hom. Imm. Obj. of Winkel) of the microscope, 

 most of the cells in the diseased region of the tissues were 

 found to contain bacteria in abundance. Figure 4 represents 

 a portion of a section of such a tissue as seen under the 

 microscope, and is magnified 800 diameters. 



Several cover-glass preparations from the pure cultures 

 of bacteria in both agar-agar and gelatine were made and 

 stained with either gentian violet or with carbafuchsin, and 

 examined with the 1-24 inch Hom. Imm. The appearance 

 of these bacteria as seen under such a high power of the 

 microscope is shown in figure 1, which represents them as 

 magnified 1500 diameters. When magnified equally, the 

 bacteria in the sections of diseased tissue will be seen to be 

 identical in appearance with those from the culture tubes. 



Not being able to identify this species of bacteria with 

 any heretofore described, I have named it 



Bacillus gossypiNA. 



Obtained by Stedman (1893) from the inside of diseased 

 cotton-bolls suffering from a lot of the seed and lint. 



3[orphology. — Short, straight bacilli, truncate with slightly 

 rounded corners, 1.5 micron long and 0.75 micron broad; 

 usually solitary, sometimes in pairs, and occasionally in 

 chains of from three to four. 



Stains readily with the usual aniline colors. 



Biological characters. — An aerobic, non liquefying (slight 

 liquefaction in old gelatine cultures), motile bacillus. 

 Forms spores. Grows at the room temperature in the usual 



