113 



The inoculated plante were gieeu. tall, and their roots 

 were abundantly supplied with tubercles. The plants not 

 ino<'ulated were yellowish, not branched, two to four inches 

 tall and there were no tubercles on the roots- There was 

 not enough for cutting with scythe or sickle. Fig 1 

 shows the contrast between typical inoculated and non- 

 inoculated plants. 



• In the fall of 1908, on poor, whitish, s?.ndy. up- 

 land soil (Xopfilk randy loam), although too late 

 for best success, a plot of crimson clover was inoculated 

 with soil from an older crimson clover field, and another 

 plot left without inoculation. These plots were not 

 harvested, but the marked ditference in appearance were 

 as follows : The inoculated plants were green, thrifty, 

 about 14 to 16 inches tall, and their roots w^ere covered 

 with tubercles; the yield was estimated at about one ton 

 of hay per acre. The plants not inoculated had no tuber- 

 cles, were yellowish, and had but on<^ or two stems per 

 plant, and wv^re not tall enough to cut. most plants dying 

 before blooming, or blooming at a height of only 2 to 6 

 inches. 



PURE CULTURES. OR ARTIFICIAL INOCULATING MATERIAL. 



In August, 1897. and in August 1898, the writer publish- 

 ed results of inoculation of crimson clover by the use of 

 pure cultures, or bottled material prepared in the lab- 

 oratory. As these bulletins (Nos. 87 and 96 of the Ala- 

 bama Experiment Station) are now out of print, some of 

 the results of these earlier tests will we referred to here. 



In both of the following tests the pure cultures used 

 was importfed from Germany under the name "Nitragin.'* 



