219 



mal temperature must be determined. To obtain the nor- 

 mal take the temperature of the animals to be tested every 

 two hours beginning at (5 a. m. and continuing until 6, 8 or 

 10 p. m. When time is important and many cases are to be 

 tested, the temperature may be taken every three hours 

 during the day. In no case where the temperature runs to 

 or above 102 degrees Fah. from morning till evening should 

 the animal be injected ; since it already has fever and the 

 characteristic reaction will not always appear. It should 

 be remembered that the normal temperature, as a rule, 

 reaches its maximum in the evening and only in very rare 

 instances in the morning. 



After getting the temperatures during the day, at 6, 8 or 

 10 p. m. the animals may be injected with tuberculin. If 

 the tuberculin, made by the Bureau of Animal Industry, is 

 used, 2 C. C. (one-half fluid drachm) is hypodermically in- 

 jected into each animal weighing 1,000 lbs. ; for bulls and 

 animals weighing over 1,00 J lbs. 3 c. c. is injected into each ; 

 for yearlings and small two-year olds use one and one-half 

 c. c; for calves 1 c. c. may be used. If Koch's or Pasteur's 

 tuberculin is used, .25 c. c. is injected into each animal weigh- 

 ing 1,000 lbs.; this must first be diluted with a one per cent, 

 solution of carbolic acid to a strength of ten per cent. This 

 may be conveniently done by pouring 5 C. C. of Koch's or 

 Pastuer's tuberculin into a perfectly clean glass vessel and 

 adding thereto 19 drachms of a one per cent, solution of 

 carbolic acid. (In making the one per cent, carbolic acid 

 solution always use boiled distilled or filtered water.) Each 

 drachm of this solution will then contain a dose for an ani- 

 mal weighing 1,000 lbs.; l.| drachms for a bull or larger 

 animal; f of a drachm for 1 and 2 year olds and ^ drachm 

 for calves. 



The hypodermic syringe should be thoroughly disinfected 

 and have a capacity of one to two di-achms. Inject the 

 tuberculin under the skin on the side of the neck or over 

 the shoulder. 



The morning following the injection begin to take the 



