G. Delgado Palacios 83 



largely its content of soluble calcium, as the following experimental 

 data among others, clearly show. 



Three ecjual weights of a given specimen of feces were treated 

 with equal volumes of distilled water. To one portion, 2 to 3 drops 

 of acetic acid sol. were added; to another, 2 to 3 drops of ammo- 

 nium hydroxid sol, and each mixture rendered homogeneous by thor- 

 ough stirring. The extracts were then filtered and the quantities of 

 calcium in the filtrates determined, with the following results : 



Calcium, CaO 



200 c.c. of fecal extract with distilled water 0.0314 gm. 



200 c.c. of fecal extract with dilute acetic acid 0.2170 gm. 



200 c.c. of fecal extract with dilute ammonia 0.0225 gm. 



In Order further to test my general conclusion, I made direct ex- 

 periments, on men, by means of rectal injections of Solutions of 

 ammoniacal and calcareous salts of volatile fatty acids. These ex- 

 periments have shown that the ammoniacal salts cause strong poly- 

 uria, whereas the calcareous induce marked hypouria. The ammo- 

 niacal injections were prepared with ammonia (3.825 gm. of NH3) 

 neutralized with butyric acid in the presence of rosolic acid and made 

 up to 100 cc. The calcareous injections were prepared with 5 cc. of 

 pure acetic acid neutralized with carbonate of lime, filtered, washed, 

 and made up to 100 cc. The daily quantity was diluted four or 

 five times with water and fractionally injected, in each case. Below 

 are recorded the results of an experiment on a given individual dur- 

 ing four periods of several days each. Throughout the experiment 

 the daily urine was collected and analyzed. Only the averages for 

 each period are given below. (The daily data for this and similar 

 experiments may be found in the book referred to above (13).) 



Calcium CaO, Volatile fatty acid 



Volume, cc. gm. (»/loNaOH sol.),cc. 



First period. Normal conditions 



Daily averages 1290.6 0.2426 24.51 



Second period. Polyurie effect of injected butyrate of ammonia 



Daily averages 2248.0 0.2989 31-56 



Third period. Hypouric effect of injected acetate of calcium 



Daily averages 1384.0 0.4503 68.7 



Fourth period. Polyuria provoked by ingested water; no injections 



Daily averages 1769.2 0.2558 25.38 



- This average does not show the füll hypouric effect of calcium acetate, 

 though its action was very strong. This period followed the period of polyuria 

 but, notwithstanding this fact, the volume of urine in the latter part of the 

 period was below normal. 



