Casimir Funk o 



Of this substance, 0.1056 gm. required, by the Kjeldahl method, 

 38.8 cc. of n/sulfuric acid sei., äquivalent to 51.43 percent of N 

 (adenin, 51.85 percent) . The combiistion of adenin, because of the 

 high nitrogen-content, gives very poor resiilts, a fact which has not 

 been mentioned in the hterature. Data obtained by the combustion 

 method are appended : 



0.1269 gm. substance gave 0.2269 gm. CO2 and 0.058 gm. H.O ; 48.7670 C* 

 5.11% H. 



O.IS79 gm. substance gave 0.2652 gm. CO, and 0.0794 gm. H^O ; 45.8i7o C 

 and s.62% H. 



Calc, for QH5N5 (135-09), 44-41% C; 3.73% H; 51.85% N. 



For further identification a small quantity of this substance was 

 converted into acetyl-adenin. One gm. of adenin was dissolved in 

 25 cc. of boihng acetic anhydride, and the Hquid poured into cold 

 water. After evaporation of the Solution, 0.95 gm. of product were 

 obtained. This material was recrystallized from dilute acetic acid, 

 the crystals washed with water, and the total yield, 0.6 gm., dried 

 in vaciio (no water of crystallization) at iio°C. The crystals de- 

 composed at 300° (uncorr.). 



0.1046 gm. of substance required, by the Kjeldahl method, 29.8 cc. of n/io 

 H,SO,; 39-88% N. 



Calc, for CsH^Ns— CO— CH3 (177.12), 39-54% N. 



The mother liquor separated from the mass of the adenin crystals 

 was precipitated with picric acid; 2.y gm. of picrate were obtained. 

 The picrate, which was very insoluble, was recrystallized from 

 water; 2.2 gm. of yellow, silky needles were obtained. These had a 

 melting point of 29i°C. (uncorr.). 



O-3478 gm. of substance lost, in vacuo, at 110° C, 0.0142 gm. H.O : 4.08%. 

 Calc, for adenin picrate, H.O : 4.70%. 



0.1154 gm. of substance gave 30.7 cc. N (753 mm., 22.5° C.) : 30-44% N. 

 Calc, for adenin picrate, CuHsNsO, (364.16) : 30-75% N. 



The initial filtrate from the adenin crystals was also precipitated 

 with picric acid. Excess of the reagent was required for this pur- 

 pose, and there was obtained 2.9 gm. of picrate identical with adenin 

 picrate. The filtrate from the picrate was shaken with 40 cc. of 

 20 percent sulfuric acid sol. and ether to remove the picric acid. 

 The aqueous sol. was neutralized with ^2 gm. of neutral lead acetate, 



