Casimir Funk 



II 



Experiments on animals. The influence of each of the above 

 fractions was tried on two pigeons. The results, for presence of 

 Vitamine in the f raction, are summarized below : 



Phosphotungstate precipitate from yeast * 



I I , 



Acetone-insoluble 

 fraction 



Picric acid precipitate 



IT 



Mercuric chlorid precipitate 



Platinic chlorid 

 precipitate 



Filtrate 



Picroionate 



Filtrate 



Acetone-soluble 

 fraction 



Filtrate 



Filtrate 



Platinic chlorid 

 precipitate 



Filtrate 



Picroionate Filtrate 



Mercuric chlorid precipitate 



Filtrate 



3. The fractionation of yeast was repeated, by a method that 

 was practically the same as that described in section 2. In this case 

 2,310 gm, of concentrated alc. extract from 90 k. of yeast were first 

 treated, on a shaking machine, with 3 1. of 10 percent sulfuric acid 

 sol. The acid extract was separated from the fat and, with phos- 

 photungstic acid, 2,496 gm. of precipitate (sHghtly moist) were 

 obtained. The acetone-insoluble fraction amounted to 213 gm. 

 The extracted fat, treated again with 2 1. of 10 percent snlfuric acid 

 sol., gave, with phosphotungstic acid, only 45 gm. of precipitate. 

 This shows that the first extraction was very nearly a complete 

 one. 



Acetone-insoluble fraction. This fraction was treated with 460 

 gm. of neutral lead acetate sol. in a mortar and the filtrate, after 

 being freed from lead, was precipitated with picric acid. The re- 

 sultant picrate (7.1 gm.) was identical with adenin picrate. The 

 filtrate was acidified with sulfuric acid and extracted with ether to 

 remove the picric acid. From this Solution sulfuric acid was re- 



* The double lines indicate the presence of vitamine. 



