Casimir Funk 13 



filtered off, and the filtrate precipitated with 50 percent phospho- 

 tungstic acid sol. The dried precipitate weighed 750 gm. and was 

 quite white in color. The precipitate was treated with acetone in a 

 mortar as previously described. After several treatments with 

 acetone the insoluble residue weighed 259 gm., considerably more 

 than in the case o£ the alc. extract of yeast. The acetone-insokible 

 residue was practically insoluble in methyl and ethyl alcohols, Chlo- 

 roform and other organic solvents, so that no further fractionation 

 could be effected in this way. 



Acetone-insoliihle fraction. The 259 gm. obtained by the fore- 

 going process, were treated, as previously described, with lead ace- 

 tate. The filtrate, freed from lead and containing a large amount 

 of acetic acid, was treated with nitric acid and then with silver 

 nitrate. It is very surprising that no precipitate was obtained. 

 Powdered baryta was added to the liquid until an alkaline reaction 

 was obtained, the precipitate decomposed with hydrogen sulfid, 

 traces of baryta removed with sulfuric acid, and the filtrate evapo- 

 rated in vaciio. The residue gave a white precipitate, with alcohol, 

 which will be investigated later. The filtrate from the silver salt, 

 freed from silver, gave only a very small amount of precipitate with 

 phosphotungstic acid. 



Acetone-soluble fraction. To the acetone sol. a sol. of neutral 

 lead acetate was added until complete precipitation occurred. The 

 precipitate was obtained by filtration and washed with 30 percent 

 acetone sol. A peculiar camphor-like smell was noticed during this 

 Operation. The filtrate, freed from lead, gave, after evaporation, a 

 crystallin substance which proved to be unchanged phosphotungstate. 

 It seems that, in the presence of a large amount of acetone, the de- 

 composition of phosphotungstates with lead acetate is incomplete. 

 To finish the process, acetone must be wholly removed by evapora- 

 tion. When lead acetate was used, all the phosphotungstic acid 

 was removed. After the removal of its excess of lead and its evap- 

 oration in vacuo, the filtrate yielded a completely crystallin residue 

 which, so far, has proved to be without power to eure beriben 



pigeons. 



5. Fractionation of the phosphotungstate precipitate 



OBTAINED FROM AUTOLYZED YEAST, BY THE USE OF ACETONE-WATER 



