Casimir Funk 



15 



a fine sieve, and shaken for i hr. with 3 1. of 25 percent acetone. 

 The insoluble part was sucked off and washed three times with a 

 total of 500 cc. of 25 percent acetone sol., dried and weighed; 400 

 grams of the precipitate dissolved. The insokible fraction was 

 again powdered and extracted, for i hr. on a shaker, with 2 l of 

 25 percent acetone sol. This time 122 gm. dissolved. The remain- 

 ing 603 gm. were powdered and extracted in the above-mentioned 

 way with 2 1. of pure acetone. The insoluble portion was separated 

 and washed with 250 cc. of acetone; 155 gm. dissolved. The ex- 

 traction was repeated with i 1. of acetone; 35 gm. dissolved. The 

 remaining 413 gm. were extracted with i 1. of 50 percent acetone sol. 

 as previously described. The insoluble residue was separated and 

 washed with 100 cc. of 50 percent acetone sol. ; 95 gm. dissolved. 

 The extraction was repeated with i 1. of the solvent and the precipi- 

 tate washed three times with 100 cc. of 50 percent acetone sol; 60 

 gm. dissolved. The remaining 258 gm. were extracted with 1,200 cc. 

 of 75 percent acetone sol., and the precipitate washed twice with 100 

 cc. of 75 percent acetone sol.; 68 gm. dissolved. The extraction 

 was repeated with i 1. of 75 percent acetone sol. and the material 

 washed twice with 100 cc. of the solvent of the same concentration ; 

 25 gm. dissolved. The extraction was repeated with 680 cc. of 75 

 percent acetone sol. for i hr. and washed twice with 100 cc. of 75 

 percent acetone sol.; 15 gm. dissolved. The insoluble residue 

 finally amounted to 150 gm. 



The degrees of Solution in the acetone media of different con- 

 centrations are indicated in the f ollowing summary : 



These results indicate that, in the case of the phosphotungstate 

 from autolyzed yeast, the residue with pure acetone amounted to 

 34 percent of the total precipitate, whereas, with acetone-water sol. 

 of different concentrations, the residue amounted to only 13.3 per- 

 cent — and might have been further diminished by additional extrac- 



