Jacob Rosenhloom 



25 



TABLE 5 



Data pcrtaining to the nitrogen, sulfnr and phosphorus contents in human 



cerebro-spinal fluid: Parts per 100 



6. On the absence of creatin and Creatinin from human 



cerebro-spinal fluid 



Examination of 200 cc. of human cerebro-spinal fluid collected 

 from different cases, failed to show any trace of creatin or Creatinin. 

 The Jaffe method was employed. 



7. A modification of Gerhardt's test for diacetic acid in urine 



The precipitate that f orms with the addition of f erric chlorid sol. 

 to urine, on testing for diacetic acid, often obscures the development 

 of the port wine color due to the presence of diacetic acid. Some 

 have recommended that this precipitate be filtered off so as to prevent 

 this difficulty. I have found, however, that if this test is carried 

 out as a contact test, similar to Heller's test for protein in urine, the 

 precipitate does not interfere but the test is made more delicate and 

 positive reactions are more striking. One simply pours about 5 cc. 

 of urine on top of 5 cc. of 10 percent ferric chlorid sol. and, if dia- 

 cetic acid or the other substances that form port wine colors with 

 ferric chlorid are present, a fine port wine color appears in the zone 

 of contact. 



