>\v l 



V s * 



PREFACE. 



These observations were made in the Children's Department of the 

 Massachusetts General Hospital with an apparatus belonging to the 

 Nutrition Laboratory. The experimental technique was exclusively 

 under the charge of Miss Alice Johnson, of the Nutrition Laboratory 

 staff. For her extraordinarily painstaking skill and fidelity we are 

 under great obligations. 



Of the numerous co-workers in this research we wish especially to 

 mention Doctors S. Morgulis and J. L. Gamble, and of the various 

 house officers and nurses, Dr. R. E. Eustis, Dr. Clifford B. Sweet, 

 and the Misses Trainer, Sullivan, and Richardson. 



The obtaining of subjects for observation was much facilitated by 

 the kindness and assistance of Dr. F. A. Washburn, the superintendent 

 of the Massachusetts General Hospital, and various members of his 

 staff. 



Our thanks are due to Dr. J. H. Wright of the Pathological Depart- 

 ment, who placed an excellent room in his laboratory at our disposal. 



We are particularly indebted to Dr. Hans Murschhauser of Dussel- 

 dorf, Research Associate of the Carnegie Institution of Washington, 

 attached to this laboratory, for his reading of the entire manuscript 

 and for numerous helpful suggestions. 



The labor of preparing much of the material in this report has fallen 

 upon Miss A. N. Darling and Mr. W. H. Leslie, whose assistance we 

 gratefully acknowledge. 



Nutrition Laboratory of the 



Carnegie Institution of Washington, 

 Boston, Massachusetts, February 7, 1914- 



3 



