CULTURAL CHARACTEES OF CHESTNUT-BLIGHT FUNGUS. 15 



The same species 1 month old gave a surface growth ratlier thick 

 and abundant, much more compact than in the younger cultures, 

 and ranging from buff yellow to white. The medium in contact with 

 the glass was marbled, the deepest color being corinthian purple and 

 the lightest ecru drab. 



Endotliia radicalis mississipjnensis. — In cultures 1 week old the 

 mycelium covered the whole surface of the medium. The aerial 

 growth was erect, fluffy, much less in quantity than in Endothia 

 radicalis and very similar to E. ^parasitica in character. The color 

 was the same as in E. parasitica but more extensive. The medium 

 was unchanged in appearance and no spore masses were seen. 



The same fungus at the age of 1 month showed a thick, rather 

 compact growth, somewhat more dense than in Endothia radicalis of 

 the same age. The color of the mycelium on the surface was cadmium 

 orange, changing to xanthine orange where in contact with the glass. 

 The spore masses were minute, rather numerous, and of a pale yellow 

 ocher color. The medium at the bottom first showed perilla purple 

 when the cultures were 20 days old, 



Endotliia gyrosa. — Cultures from mycelium when 1 week old 

 covered the whole surface of the medium. The growth was very 

 thin, loose, and fluffy, pure white except at the point of inoculation 

 and where in contact with the glass. At these points it was a light 

 ochraceous buff with a faint suggestion of purplish lilac. The color 

 of the medium was unchanged and no spores were produced. 



The same fungus when 1 month old showed an abundant thick 

 growth with a cottony tomcntose pulvinate surface. The surface 

 mycelium was capucine yellow, changing to xanthine orange where 

 in contact with the glass. The color of the medium was marbled, 

 rangmg from lumiere green to apple green. The line marking the 

 boundary between the mycelium and the culture medium on the sides 

 of the flask was sooty black. No spores were found. 



CULTURES ON CORN MEAL. 



Endothia 'parasitica. — In cultures 1 week old the growth on corn 

 meal covered about one-half of the surface of the medium. The 

 outer margin was pure white, the remainder buff' yellow below, with 

 a superficial white growth above and the medium uncolored. A few 

 smaU pustules with spore masses occurred near the point of 

 inoculation. 



Cultures 1 month old showed a compact growth nearly smooth on 

 the surface. The supei-ficial mycelium was pale orange yellow. The 

 pale yellow ocher sporo masses were minute, very numerous, and 

 nearly covered the surface. The medium was slightly greenish about 

 the sides of the flask just beneath the mycelium. 



[Cir. 131] 



