BOTANICAL GAZETTE 



SEPTEMBER 



between ethylene treated and untreated tissue; for, as has been 

 stated, a given culture was not divided into the two portions until 

 72 hours before the end of the cultural period. Considering the 

 unavoidable variations which must enter into different cultures, 

 the treated and untreated pair of samples from one culture should 

 therefore be more comparable than any other combination; for 

 example, the treated sample of one culture and the untreated of 

 another. Samples I and II, afterward IV and V, VI and VII, 

 etc., are directly comparable. 



TABLE I 



Total alcohol-ether soluble substances in the ethylene treated and 

 untreated tissue 



No. of 

 sample 



Percentage 

 of alcohol- 

 ether 

 soluble 



Percentage 

 of alcohol- 

 ether 

 insoluble 



Percentage 

 of total 

 solids 



Percentage 

 of alcohol- 

 ether 

 soluble 



Percentage 

 of alcohol- 

 ether 



insoluble 



Percentage 

 of total 

 solids 



I 



V 



VII.... 

 XIII. .. 

 XV ... . 

 XIX. . 

 XXI. . . 

 XXIII . 



Mean 



II 



IV 



VI 



XII 



XIV 



XVIII (a) 

 XVIII (b) 



67.63 

 68.80 

 67-43 



32-37 

 31.20 



32.57 

 35-02 

 32-40 

 3 J -36 

 3 r -34 



8.27 



67.70 



8.48 



Table I gives the results of the separation of the samples into 

 alcohol-ether soluble and insoluble portions. Two facts are note- 

 worthy: first, that the soluble substances are more abundant .in 

 the ethylene treated than in the untreated tissue, a difference 

 amounting to about 8 per cent; and, secondly, the water content of 

 the two tissues is practically the same, being about 91.5 per cent. 

 To what substances this difference is due should become clearer 

 from the tables to follow. 



Table II shows the amount of reducing sugars in the alcohol- 

 ether soluble fraction before and after hydrolysis, and also the redu- 

 cing power of the insoluble fraction after hydrolysis. These latter 

 data cannot be expressed, even approximately, in percentages, 



