204 



BOTANICAL GAZETTE 



[SEPTEMBER 



developed in the untreated epicotyl. This anatomical difference 

 agrees with the findings of Kaufmann (18) for lupine seedlings 

 treated with ether. From a chemical viewpoint the difference 

 seems sufficient to account for the greater power of reduction of the 

 untreated tissue after hydrolysis, inasmuch as mechanical tissue 

 contains a large proportion of rather easily hydrolyzable carbohy- 

 drates. That these polysaccharides are not completely hydrolyzed 

 by the 2 . 5 hours' hydrolysis is shown by comparing samples 

 XIII (a) and XIII (b), in which the hydrolyzing time of the latter 

 was doubled. 



• Hydrolysis s hours. 



Table III gives the results of "crude fiber" determinations. 

 "Crude fiber" obviously is a mixture of a large number of sub- 

 stances, such as unhydrolyzed protein, cellulose and other poly- 

 saccharides, etc. However, this fiber must be largely cellulose 

 and protein. Less crude fiber was found in the treated tissue. 

 The approximate percentage of cellulose was estimated by sub- 

 tracting from "crude fiber " the total protein present before hydrol- 

 ysis, minus the protein rendered soluble by the acid hydrolysis. 

 By this method relative differences, at least, should be shown, and 

 the results indicate that the treated tissue had about 3 per cent 

 less cellulose than the control. 



In table IV are shown the total nitrogen and the ammonia and 

 amino nitrogen, before and after hydrolysis, in the alcohol-ether 

 soluble fraction, and the total nitrogen in the alcohol-ether insoluble 

 fraction. 



The total nitrogen figures for the former fraction do not show 

 a difference between the treated and untreated tissues. Both the 



