1915] 



IJARl 'E I '— E Til 1 -LENE 



205 



formol titration and the Van Slyke methods show a higher amino 

 nitrogen content in the treated tissue before hydrolysis, but after 

 hydrolysis the difference does not appear. (Determinations after 

 hydrolysis were made only by the Van Slyke method.) Since the 

 increase of alpha-NTT nitrogen, by hydrolysis, is somewhat more 

 in the untreated tissue, it suggests a less amount of polypeptides 

 in the treated tissue, a situation for the soluble nitrogenous sub- 

 stances corresponding to that of the soluble carbohydrates of this 

 fraction. The ammonia-nitrogen results are very variable and no 

 conclusions can be drawn from them. 



TABLE IV 



Nitrogen 



♦Obtained by the formal-titration method. 



The total nitrogen of the alcohol-ether insoluble fraction is 

 less in the treated tissue. By employing the factor 6.25 to the 

 nitrogen figures, it is found that the proteins are about 3 per cent 

 less in the treated tissue. Although the results at first seem some- 

 what inconsistent, they no longer appear so when treated and 

 untreated samples of the same culture are compared. 



