29S BOTANICAL GAZETTE [april 



a suction of 2 cm. of mercury with an aspirator. Molds continued 

 to grow on the surface of this solution. It had a slightly darker 

 color than iA. 



Solution iC. — The ether used in extracting iB was allowed to 

 evaporate spontaneously. The solid remaining was reddish- 

 brown, and only partially soluble in water. This solution consists 

 of 1600 cc. of redistilled water, and all the residue from the ether 

 extraction that would dissolve in that quantity of water at 40 C. 

 No molds grew on this solution, and no scum or turbidity or other 

 evidence of bacterial activity appeared. 



Preparation iD. — This was the solid remaining from filtering 

 1 A. This solid was ground with an equal volume of sand. 



Solution 2 A. — This was the distillate under reduced pressure 

 (2 cm. pressure), at 40 C, of the liquid and solid products of the 

 decay of 1000 gm. of Nymphaea rhizome in redistilled water. This 

 was a clear liquid having the appearance of water. No molds 

 grew upon it, and it showed no evidence of bacterial activity. 



Solution 2B. — The solid remaining from the distillation of 

 2 A was dried in an oven at 30 C. and then ground in a mortar. 

 It was then black powder. This was extracted in a Soxhlet appa- 

 ratus with ether. When the ether was allowed to evaporate spon- 

 taneously, a sticky, semi-solid, reddish-yellow substance remained. 

 This was only partially soluble in water. This solution represents 

 1600 cc. of redistilled water, with all of the ether extract that would 

 dissolve in it at 40 C. It had a light reddish-yellow color. No 

 molds grew upon it, and no evidence of bacterial activity appeared. 



Solution 2C. — The solid remaining from the extract of 2B 

 was exposed to air until all odor of ether had disappeared. It 

 was then extracted for 2 hours with 1600 cc. of redistilled water. 

 It was perfectly clear. No molds or evidence of bacterial growth 

 appeared. 



Preparation 2D. — This represents the solid remaining from the 

 extract of 2C, ground in a mortar with an equal volume of sand 

 to form a soil. 



Solution jA. — One kg. of fresh Nymphaea rhizome was cut into 

 pieces, ground in a meat grinder, and the juice pressed out in a 

 fruit press. The solid remaining was extracted with ether, and 



