22 CAUSE AND NATURE OF CROWN-GALL 



merits were of various sizes and ages ; some were fresh and suc- 

 culent, while others were dry and partially decayed. Plot III 

 was treated in the same manner as plot II, with the exception 

 that the minced galls were mixed with an equal weight of flowers 

 of sulphur before placing in the soil. Plot IV was also treated 

 the same as plot II, with the exception that the galls were mixed 

 with one-half their weight of Milestone (copper sulphate ) and 

 enough water added to partially dissolve the fungicide. 



At the end of eleven months the seedlings were harvested and 

 the results tabulated. At this time there were twenty-seven 

 seedlings in plot I, twenty-nine in plot II, thirty-three in plot 

 III, and twenty-two in plot IV. The results are represented 

 in the following table, and the position of the diseased seedlings 

 is schematically shown in Fig. 3 : 



N ° of Treatment. 



plot. 



No. of diseased 

 trees. 



I ' Untreated ° 



II Several pounds of minced galls 16 



III 1 Minced galls and sulphur '7 



IV Minced galls and bluestone 



At the time of harvesting the seedlings were from two to three 

 feet high and the stems a half inch or less in diameter. In 

 most instances the galls appeared at the crown and were from 

 one to three inches in diameter (Fig. 4). 



* In digging the seedlings from plot III it was observed that 

 the sulphur was still abundant in the soil. In several instances 

 galls were found that were partially surrounded by flowers of 

 sulphur, and in all cases they were as vigorous as those that 

 grew in the non-sulphured soil. In the sulphured plot one 

 more diseased tree was found than in plot II, where no fungi- 

 cide was used. The results of my experiments with sulphur 

 seem to be in accord with those of Selby," but are at variance 

 with those of Halsted. 1 ' 5 



24 Bull. Ohio Agr'l Exp. Sta., 104, 211. 



25 Kept. Bot. Dept. N. J. Agr'l Exp. Sta. 1896, 414. 



