MICHIGAN ACADEMY OF SCIENCE. 93 



UNSEALED CULTURES GROWN IN DARK. 



Cultures grown in the dark showed veiw slight dififereuces over 

 those in the light. The rate of growth was slightly faster in the 

 majority of cases and the colors varied a little in shade but no 

 other differences were noted. The spores appeared about the same 

 time and were similar in size and shape. 



SEALED CULTURES. 



The growth of the hermetically sealed cultures indicated that 

 the organism does not thrive under anaerobic conditions, as the 

 growth was very slow, ceasing after the ninth day in most, cases. 



From the examination of the above data it can be seen that 

 the fungus behaves very nearly the same on all kinds of culture 

 media. The most important fact bronght out in the experiment 

 was the rapidity with which spores are produced. The usual time 

 was two days and it can be seen that a fungus which fruits so 

 rapidly must of necessity be hard to control. 



The best culture media of those tried were cucumber agar, 

 parsnip and carrot plugs, particularly the latter. These furnished 

 an excellent media for all kinds of work since great numbers of 

 spores were produced within the space of two or three days. 



The slight variations in color noted are not sufficient to Avarrant 

 any particular notice since they were but shades of olive green. 



The rate of growth was the most variable factor together with 

 the fact that the fungus evidently does not thrive anaerobically, 

 judging from the checking of growth in all the sealed tubes. The 

 spore form does not seem to vary appreciably on different media 

 nor does the character of the mycelium undergo any radical al- 

 teration. 



DESSICATION EXPERIMENTS WITH SPORES. 



A number of sterile cover glasses were used in this experiment, 

 each one being smeared with a solution of spores of Claclosporium 

 cucumerin'um in distilled water. These were allowed to dry in the 

 light at room temperature and were then broken up into small 

 pieces, the fragments being planted in tubes of cucumber agar at 

 intervals of 24 hours. 



Five tubes were inoculated each day and the experiment was 

 continued until the spores no longer showed the power of germ- 

 ination. 



This was begun on December 24, and continued up to January 19. 



