MICHIGAN ACADEMY OF SCIENCE. 141 



SOME CULTURAL CHARACTERISTICS OF TESTALOZZIA 



FUNERA DESM. 



BY P. V. SIGGERS. 



A c'omjiarative stiidv* of vegetative growth was made on several 

 kinds of agar and upon gelatin. Pine, carrot, corn meaL dextrose 

 and synthetic agars were nsed. The cnltnres were made Jan. 

 16, 1915, and they represent the vegetative growth that appears 

 in 14 days. Cnltnres were repeated so that representative cnltnres 

 conld be obtained. 



Corn meal agar. Upon corn meal lateral growth (PI. XI A) is 

 comparatively slow. In the alloted time the distance covered was 

 but two-thirds the distance from the center to the sides of the dish. 

 There was little snperficial growth and this type of growth con- 

 sisted in nothing more than small, erect, simple branches from the 

 snrface of the agar. 



Diifipar'.^ Synthetic agar amd 1/20% peptone: Upon this agar 

 the fungns spreads ont in fan-shaped concentric areas (PI. XI R). 

 Three or fonr irregnlarly concentric circles are formed by a dense, 

 snpei'ficial growth. The snperficial mycelium remained unchanged 

 in color and was dense enough to produce a decidedly bush effect. 



Dnggar's Sj/nthetic agar rt/?^ 1/2% pe^ptone: Upon this agar 

 there is a streaked appearance (PI. XI C.) which is produced by 

 the mycelium growing in thin fan-shaped areas. 



Carrot agar: On carrot agar there is a density and a regularity 

 of growth (PI. XII A) which was not in evidence on the other 

 media. An abundant snperficial growth completely hides the sur- 

 face of the agar. The erect aerial mycelium stood from 2 to 3 mm. 

 above the surface of the agar. 



Dextrose agar: Growth upon dextrose agar is characterized by 

 rapid lateral (PI. XII B) growth. The fnngns spreads rapidly 

 over the snrface in irregular fan-shaped areas and scattered snper- 

 ficial masses a])i)ear npon the surface of the cnltnre. This snper- 

 ficial growth has a tendency to form circular cell aggregations. In 

 snch regions many anastomosing filaments are fonnd. 



Beef gelatin: On gelatin. (PI. II C) growth continues for a 

 few days and stops. In that period hardly a third of the distance 

 from the center to the edge of the dish is covered. Snperficial 

 groAA-th is abundant and there are no concentric circles which indi- 

 cate changes in rate of growth. There is no liquefaction. 



♦Cryptogamic Laboratories, University of Michigan, Ann Arbor. 



