MICHIGAN ACADEMY OF SCIENCE. 279 



and Saxony, Germany. The same herbarium also contains specimens 

 collected in 1889 in Manhattan, Kansas, — probably the first specimens 

 collected in this country. The Michigan Agricultural College herbarium 

 contains sijecimens collected by C. F. Wheeler in the local Botanical 

 Garden in 1898. Other specimens contained in the U. S. Dept of Agri- 

 culture herbarium were collected at Houlton, Me., (1906), Arlington 

 Farm, Va., (1907), Germantown, Md., (1908), and Philadelphia, Pa., 

 (1909), besides one specimen on TrifoUum sp. collected at Potomac 

 Flats, Va., (1890). 



The records* of the Plant Disease Survey of the Bureau of Plant 

 Industry report that the disease was common and injurious in certain 

 fields in Connecticut in 1903; widely distributed in Tennessee in 1905; 

 caused great loss of young clover in West Virginia in 1906, but was un- 

 important in 1910, 1911 and 1912. It was reported from Minnesota 

 for the first time in 1910.** 



The collections of the herbarium of the U. S. Department of Agri- 

 culture also record the disease on four specimens of alfalfa. Slides 

 from some of this material were kindly sent for examination. It at once 

 became evident that the fungus on alfalfa is not the same as that on 

 red clover. Slide mounts of spores on material collected in Philadelphia, 

 Pa., and Arlington Farm, Va., (Turkestan alfalfa) contained spores 

 which in shape and color are the same as the spores of Macrosporium 

 sarcinaeforme Cav. on clover, but they are smaller in size and decidedly 

 warty. On the strength of these morphological differences, the author 

 believes that the fungus on alfalfa is a new species of Macrosporium, 

 and is surely not identical with M. sarcinaeforme Cav. on red clover. A 

 search of the literature has failed to reveal a species of Macrosporium 

 which conforms with this Macrosporium on alfalfa. A more complete 

 description of this fungus will be given at another time. 



SIGNS OF THE DISEASE. 



On the leaves: 



Usually within 21-36 hours after inoculation, a minute light brown 

 spot just visible with a hand lens, appears on the leaf surface as a 

 result of the penetration of the fungus. After three days, it has attained 

 a size of 2-3 mm. diameter but has no definite shape. After this it en- 

 larges rapidly, and the typical concentric markings begin to form. The 

 center of the well developed spot is darkest and very distinct. Around 

 this appear the alternately lighter and darker concentric rings. The 

 darker rings are sepia to dark brown in color, the lighter ones, ochre to 



*Obtained through the courtesy of Mr. N. L. Lacey. 



**The author found the disease quite common on the N. D. Agricultural College farm 

 in the fall of 1916. 



