32i 



NINETEENTH REPORT. 



APPENDIX. 



Krakover Clover Leaf Spot. 



Formulae for Media tised : 



Nutrient dextrose agar. 



Plain nutrient agar plus 5% 

 dextrose. 



Full nutrient sol. Kundson. 



1000 cc. water. 

 1 gm. KNO,. 

 0.5 gm. KH,PO,. 

 0.25 g. MgSO^. 

 0.002 g. FeXl,;. 

 5 g. cane sugar. 



Coons' synthetic Sol. 



Stock m/5 

 MgSO^, 2.466 g. + 50 cc. H,0. 

 KH,PO^ 1.36 g. + 50 cc. H„0. 

 Asparagin 1.33 + 50 cc. HoO. 

 ■ Maltose 3.6 + 50 cc. H.,0. 



For 100 cc. Synthet. Sol. 



1 cc. of m/5 MgSO^ and Aspar. 



5 cc. of each of others. 



88 cc. of water. 



Steam 3 consecutive days. 



Coons' synthetic agar. 



Above solution plus 1.2% agar. 

 Steam 3 consecutive days. 



Rice. 



Add 3 volumes of tap water to 1 



volume of rice. 



Autocl. 15 ll)s., 20 minutes. 



Corn meal. 



Corn meal with about equal vol- 

 ume of taj) water. 



Autocl. 15 lbs. 20 minutes. 



Potato plugs. 



Wash and peel a firm tuber. Cut 

 into wedge shaped plugs, and 

 place in tubes containing short 

 pieces of glass rod and few cc. 

 of water. Autocl. 20 lbs., 20 



min. 



The following other vegetable 

 plugs were prepared in the same 

 manner as the potato: Carrot, 

 parsnip, sugar beet, red table 

 beet. 



Sorghum stems. 



Cut stems into pieces 2-3 inches 

 long. Put into tubes containing 

 little water. Autocl. 15 lbs., 20 

 minutes. 



Clover stems. 



Same as sorghum. 



Bean Pod. 



Mature pod of wax bean put in 

 tube witli little water. Autocl. 

 15 lbs., 20 minutes. 



Clover juice. 



200 g. of finely cut clover plants 

 (leaves and stems) 1000 cc. tap 

 water. Boil one hour, adding 

 water occasionally to make up 

 for loss by evaporation. Filter 

 through cheesecloth and let stand 

 a few hours until fine settlement- 

 settles to bottom. Filter through 

 paper. Autoclave 15-20 lbs. for 

 15 minutes. 



