MICHIGAN ACADEMY OF SCIENCE. 



325 



Clover juice agar. 



Add 1.2% agar to above liquid. 

 Clear and filter. Autocl. 15-20 

 lbs. for 15 minutes. 



Prune juice agar. 



120 g. prunes, 1000 ce. water. 

 Boil in steamer two hours. Filter 

 through cheesecloth. Restore to 

 original volume. 



12 g. agar. Boil in steamer two 

 hours. Clear and filter. Autocl. 

 20 lbs., 20 minutes. 



Corn meal agar. 



Three tablespoons ful cornmeal, 

 1000 cc. water. Cook in double 

 boiler at 60° C. for three hours. 

 Decant, restore to original vol- 

 ume. 



15 g. agar. Clear and filter. 

 Autocl. 15 lbs., 20 minutes. 



Oat agar. 



75 g. Quaker oats, 1000 cc. 



water. Cook 

 two hours. 



in double boiler 

 Strain through 



cheesecloth, squeezing out the 

 slimy matter. 



10 gms. agar. Boil one liour. 

 Autocl. 15 lbs., 20 minutes. 



Thaxter-Hard potato agar. 



250 g. sliced potato, 1000 cc. 

 water. Cook one-half hour in 

 steamer. Decant and restore to 

 original volume. 



20 g. of glucose. 



30 g. of agar. 



Cook in steamer three hours. 



Clear and filter. 



Autocl. 15 lbs., 20 minutes. 



Nutrient glucose agar. 



to gms. glucose, 1000 cc. dis- 

 tilled water, 3 g. of NaCl., 20 g. 

 peptone, 5 g. beef extract, 30 g. 

 of agar. Clear and filter. 

 Autocl. 20 lbs., 20 minutes. 



Plain nutrient agar. 



1000 cc. of water, 15 g. agar, 3 

 g. of beef extract, 10 g. peptone 

 titrate. Clear and filter. 

 Autocl. 15 lbs., 15 minutes. 



