﻿Commissioner 
  of 
  Agriculture. 
  551 
  

  

  The 
  properties 
  of 
  tuberculin 
  O 
  are 
  very 
  similar 
  to 
  those 
  of 
  

   tubercle 
  antitoxin, 
  except 
  that 
  TO 
  causes 
  no 
  abscesses. 
  Tuber- 
  

   culin 
  E 
  has 
  the 
  important, 
  property 
  of 
  rendering 
  the 
  system 
  im- 
  

   mune 
  to 
  the 
  different 
  components 
  of 
  the 
  tubercle 
  bacillus. 
  Koch 
  

   demonstrated 
  this 
  quality 
  of 
  TE 
  by 
  a 
  large 
  number 
  of 
  experi- 
  

   ments. 
  Further 
  tests 
  by 
  other 
  investigators 
  have 
  thrown 
  much 
  

   doubt 
  upon 
  the 
  high 
  immunizing 
  efficiency 
  of 
  this 
  substance. 
  

  

  II. 
  Directions 
  for 
  Using 
  Tuberculin. 
  

  

  I. 
  The 
  regulations 
  prescribed 
  for 
  many 
  years 
  by 
  the 
  Bureau 
  of 
  

   Animal 
  Industry 
  for 
  the 
  use 
  of 
  tuberculin 
  in 
  testing 
  cattle 
  are 
  as 
  

   follows 
  :^ 
  The 
  febrile 
  reaction 
  in 
  tuberculous 
  cattle 
  following 
  the 
  

   subcutaneous 
  injection 
  of 
  tuberculin 
  begins 
  from 
  six 
  to 
  ten 
  hours 
  

   after 
  the 
  injection, 
  reaches 
  the 
  maximum 
  nine 
  or 
  fifteen 
  hours 
  

   after 
  the 
  injection, 
  and 
  returns 
  to 
  normal 
  eighteen 
  to 
  twenty-six 
  

   hours 
  after 
  the 
  injection. 
  

  

  In 
  conducting 
  the 
  test 
  the 
  follo"\^'ing 
  course 
  is 
  recommended 
  to 
  

   those 
  who 
  wish 
  to 
  obtain 
  the 
  most 
  accurate 
  results 
  : 
  

  

  1. 
  Begin 
  to 
  take 
  the 
  rectal 
  temperature 
  at 
  6 
  a. 
  m., 
  and 
  take 
  it 
  

   every 
  hour 
  thereafter 
  until 
  midnight. 
  2. 
  Make 
  the 
  injection 
  at 
  

   midnight. 
  3. 
  Begin 
  to 
  take 
  the 
  temperature 
  next 
  morning 
  at 
  6 
  

   o'clock, 
  and 
  continue 
  as 
  on 
  the 
  preceding 
  day. 
  

  

  To 
  those 
  who 
  have 
  large 
  herds 
  to 
  examine 
  or 
  who 
  are 
  unable 
  

   to 
  give 
  the 
  time 
  required 
  by 
  the 
  above 
  directions 
  the 
  following 
  

   shortened 
  course 
  is 
  recommended 
  : 
  

  

  1. 
  Begin 
  to 
  take 
  the 
  temperature 
  at 
  8 
  a. 
  m., 
  and 
  continue 
  every 
  

   two 
  hours 
  until 
  10 
  p. 
  m. 
  (omitting 
  at 
  8 
  p. 
  m. 
  if 
  more 
  convenient). 
  

   2. 
  Make 
  the 
  injection 
  at 
  10 
  p. 
  m. 
  3. 
  Begin 
  to 
  take 
  the 
  rectal 
  

   temperature 
  next 
  moniing 
  at 
  G 
  or 
  8 
  o'clock, 
  and 
  every 
  two 
  hours 
  

   thereafter 
  until 
  6 
  or 
  8 
  p. 
  m. 
  

  

  Each 
  adult 
  animal 
  should 
  receive 
  2 
  c.c. 
  (about 
  30 
  minims) 
  of 
  

   the 
  undiluted 
  tuberculin 
  as 
  it 
  is 
  sent 
  from 
  the 
  laboratorv. 
  Year- 
  

  

  25 
  Dr. 
  de 
  Schweinitz. 
  Year-book, 
  U. 
  S. 
  Department 
  of 
  Agriculture, 
  1898, 
  

   p. 
  111. 
  

  

  