﻿Commissioner 
  of 
  Agriculture. 
  509 
  

  

  of 
  the 
  Bureau 
  of 
  Animal 
  Industrj 
  (1896-7) 
  page 
  269, 
  which 
  is 
  

   as 
  below: 
  

  

  " 
  The 
  method 
  of 
  diagnosing 
  rabies 
  which 
  we 
  have 
  followed 
  

   and 
  which 
  the 
  experience 
  of 
  pathologists 
  has 
  shown 
  to 
  be 
  the 
  best, 
  

   is 
  the 
  subdural 
  inoculation 
  of 
  rabbits 
  with 
  a 
  suspension 
  of 
  the 
  

   brain 
  or 
  spinal 
  cord 
  of 
  the 
  suspected 
  animal. 
  The 
  subdural 
  inocu- 
  

   lation 
  ^\dth 
  the 
  brain 
  tissue 
  of 
  rabid 
  animals 
  was 
  first 
  demonstrated 
  

   by 
  Pasteur 
  to 
  be 
  more 
  reliable 
  and 
  more 
  rapid 
  in 
  its 
  results 
  than 
  

   the 
  subcutaneous 
  injections. 
  The 
  procedure 
  is 
  simple. 
  The 
  brain 
  

   of 
  the 
  suspected 
  animal 
  is 
  removed 
  with 
  asceptic 
  precautions, 
  as 
  

   soon 
  as 
  possible 
  after 
  death. 
  A 
  small 
  piece 
  of 
  the 
  brain 
  or 
  spinal 
  

   cord 
  is 
  placed 
  in 
  a 
  sterile 
  mortar 
  and 
  thoroughly 
  ground 
  with 
  a 
  

   few 
  cubic 
  centimeters 
  of 
  sterilized 
  water 
  or 
  bouillon. 
  This 
  forms 
  

   the 
  suspension 
  to 
  be 
  injected. 
  The 
  hands 
  of 
  the 
  operator 
  and 
  all 
  

   instruments 
  are 
  carefully 
  disinfected. 
  The 
  rabbit 
  is 
  etherized, 
  

   the 
  hair 
  clipped 
  from 
  the 
  head 
  between 
  the 
  eyes 
  and 
  ears, 
  and 
  the 
  

   skin 
  thoroughly 
  washed 
  and 
  disinfected. 
  A 
  longitudinal 
  incision 
  

   is 
  then 
  made, 
  the 
  skin 
  and 
  sulxiutaneous 
  tissue 
  held 
  back 
  by 
  means 
  

   of 
  a 
  speculum, 
  a 
  crucial 
  incision 
  is 
  made 
  in 
  the 
  periosteum 
  on 
  one 
  

   side 
  of 
  the 
  median 
  line 
  to 
  avoid 
  hemorrhage 
  from 
  the 
  longitudinal 
  

   sinus, 
  and 
  the 
  four 
  parts 
  of 
  the 
  periosteum 
  reflected 
  or 
  pushed 
  

   back. 
  By 
  the 
  aid 
  of 
  a 
  trephine 
  a 
  small 
  button 
  of 
  bone 
  is 
  easily 
  

   removed, 
  leaving 
  the 
  dura 
  mater 
  exposed. 
  With 
  a 
  hypodermic 
  

   syringe 
  a 
  drop 
  or 
  more 
  of 
  the 
  rabid 
  brain 
  suspension 
  is 
  injected 
  

   beneatli 
  the 
  dura, 
  the 
  periosteum 
  is 
  replaced, 
  the 
  skin 
  carefully 
  

   sutured 
  and 
  disinfected, 
  and 
  the 
  rabbit 
  returned 
  to 
  its 
  cage. 
  

  

  " 
  As 
  soon 
  as 
  the 
  influence 
  of 
  the 
  anseesthetic 
  has 
  passed 
  off 
  the 
  

   rabbit 
  sho^vs 
  no 
  appearance 
  of 
  discomfort. 
  If 
  the 
  operation 
  is 
  per- 
  

   formed 
  in 
  the 
  forenoon 
  the 
  animal 
  partakes 
  of 
  its 
  evening 
  meal 
  

   with 
  the 
  usual 
  relish. 
  The 
  inoculation 
  wound 
  heals 
  rapidly, 
  and 
  

   the 
  rabit 
  exhibits 
  every 
  appearance 
  of 
  being 
  in 
  perfect 
  health 
  imtil 
  

   the 
  beginning 
  of 
  the 
  specific 
  symptoms, 
  which 
  occur 
  ordinarily 
  

   in 
  from 
  fifteen 
  to 
  thirty 
  days 
  after 
  the 
  inoculation. 
  Occasionally 
  

   the 
  symptoms 
  appear 
  earlier 
  than 
  fifteen 
  days, 
  and 
  in 
  some 
  cases 
  

   the 
  rabbits 
  are 
  not 
  attacked 
  for 
  from 
  one 
  to 
  three 
  months." 
  

  

  The 
  symptoms 
  following 
  inoculation 
  were 
  the 
  same 
  as 
  described 
  

  

  by 
  Dt. 
  Moore, 
  in 
  the 
  above 
  named 
  article, 
  as 
  follows: 
  

  

  " 
  The 
  symptoms 
  following 
  the 
  inoculations 
  have 
  l)een 
  quite 
  uni- 
  

   form, 
  the 
  only 
  pronounced 
  difference 
  being 
  in 
  the 
  length 
  of 
  time 
  

   the 
  rabbits 
  lived 
  after 
  the 
  initial 
  manifestations 
  of 
  the 
  disease. 
  

   The 
  fact 
  should 
  be 
  clearly 
  stated 
  that 
  rabbits 
  do 
  not 
  ordinarily 
  

  

  