106 Missouri Agricultural Report. 



tion of cholera, on a farm several miles from the Station. The 

 feeding of the diseased organs had no bad effect on the vaccinated 

 pigs mentioned, and none of the other vaccinated animals exposed 

 to them have shown any signs of illness up to the time of issuing 

 this report. 



Lot 2 — Lot 2 included 10 pigs, weighing from 40 to 60 pounds, 

 eight of these were injected with 20 c. c. of the protective serum 

 (B. A. I.), and simultaneously with 2 c. c. of fresh virulent hog 

 cholera blood. A larger quantity of diseased blood was used in 

 this case to determine the protective power of the serum, with 

 varying doses of the infection. This blood was shown to be viru- 

 lent when used on pigs not protected with serum. These inocula- 

 tions were made October 22. As in lot I, two untreated check pigs 

 were placed in the pen. Up to November 16, twenty-five days af- 

 ter inoculation, no disease was observed in any of the pigs. On 

 this date one of the pigs which had not been vaccinated became 

 sick and died on November 24th, after an illness of eight days. 

 The symptoms and post-mortem examination showed the presence 

 of cholera. On November 22nd, the second check pig became sick 

 and was killed December 2. Post-mortem examination showed hog 

 cholera lesions. The check pigs, it is certain, did not contract the 

 disease from the pigs of the same pen that had been injected with 

 both serum and diseased hog cholera blood — but probably got the 

 infection from an adjoining pen (see lot 4) . December 10 all the 

 vaccinated pigs were doing well. None had shown any signs of 

 illness. On this date two sick pigs from an infected herd were 

 put in this pen. Two days later one of them died. The other, 

 after several days' illness, recovered. This exposure caused no ill 

 results to the vaccinated pigs. On January 24 two of the vaccinated 

 hogs of this lot were fed diseased viscera from the same source as 

 that mentioned under lot 1. No ill effects resulted to the animals 

 fed, nor to the other pigs exposed to them. All of these vaccinated 

 pigs are, at the present time, alive and in thrifty condition. 



Lot 3 (pen 4) contained eight pigs — weight 40 to 60 pounds. 

 October 22, each pig was injected with 20 c. c. serum from same 

 source as that used in lots 1 and 2, but the infected hog cholera 

 blood that was injected at the same time was not freshly collected, 

 but had been preserved for some time in sealed glass tubes. This 

 was sent to us by Dr. Niles of the Bureau ; but I was advised later 

 by Dr. Dorset of the Bureau not to use this on account of an un- 

 favorable report that had been received. The unfavorable results 

 were at this time thought to be due to changes that had taken 



