N..V., 1916.1 Y. KUWADA.— SOME PECULTARrriES OBSERVED. 357 



the fresh materials as it is covered witli chromatophores and 

 oil drops. But ill microtome vSections it was found to he situated 

 ill the centre of the body. The body is 15-1)// in length and 

 11-7 ft ill breadth. The lengtli of cilia is longer than that of the 

 body. For instance I measured cilia of a swarmspore to be 

 IG [i and its body 12 n in length. 



As to the chemical nature of the cell wall Frank'' was not 

 nble to demonstrate the cellulose substance. My observation 

 confirms his result so far as cellulose nature of the wall is 

 concerned. The tests for the latter with chlorzinc iodide 

 and staining with eongored were negative. The iodine and 

 sulphuric acid gave brown colour, but not blue colour as is 

 typical w^ith cellulose. Corallin and anilin blue were also em- 

 ployed without effects. The red staining with ruthenium red was, 

 on the other hand, very beautiful. Also methylen blue, fuchsin, 

 gentian violet and safranin stained well. From these reactions 

 and stainings it may be concluded that the cell wall is a 

 geratinous membrane of the nature of pectin substance, and 

 neither of cellulose nor of callose. 



The cell division takes place lengthwise, and the nuclear 

 division seems to occur earlier than that of pyrenoid. In the 

 fresh culture the mother cell divides into two or successively 

 into four or even eight daughter cells or swarmspores. In the 

 old culture they used to take form of so called glaeotystis. 

 We find in this form 16 or 32 cells of the same size or some- 

 times of the different size w^ithin a mother cell membrane. 



Summary'.— In the foregoing pages I have reported : 



1. The behaviors of the organism differ in the different 

 media of the culture. 



2. The swarmspores respond to diffuse light negatively, un- 

 less it is not too weak. 



3. The sense of reaction to light may be subjected to change 

 by certain external agents. 



4. The convection current due to the difference of tempera- 

 ture in the different parts of the culture medium gives rise to a 



1) Bot. Ztg. Ed. 62. 1904. 



