12 THE BOTANICAL MAGAZINE. LVol. xxii. No. 252. 



Species closely allied to Chlamydomucor Oryzae on account 

 of the scantiness of the sporangium formation/^ they must be 

 regarded hereafter as entirely different ^species. 



Moreover, we have to mention here as the distinctive 

 characters of Rhizopus oligosporas and Ch. Oryzae that the 

 cultures of the former on steamed rice become brownish yellow 

 in colour, while those of the latter take yellowish coloration. 



(2) " Shiro-koji "—Yeast. 

 When a small piece of the " Shiro-koji " is added to sac- 

 charine solutions, it induces very active fermentation, and from 

 the white pasty deposit formed at the bottom of the flask, the 

 species is easily separated. The mash made by using the 

 " Shiro-koji " contains also a large number of cells belonging 

 to this yeast. 



Young cells are usually globoid in shape, mostly separated 

 from one another. The average size of the cells is 5 to 8 fJ. in 

 diameter, and they contain one or more vaciioles, unequal in 

 their size and enclosed in hj' aline protoplasm. In older cultures 

 we often find long sausage-shaped cells. 



The colonies are at first visible as small points, which 

 gradually grow to round, conspicuously dome'-shaped, yellowish 

 white clumps. There is no limit to the size of the colonies, but 

 at their surface no wrinkling is to be found. 



It is very characteristic of this yeast, that the streak culture 

 on the gelatine shows the liquifaction of the medium within a 

 few days. 



Whenever the fermentations were carried on, and even till 

 they were quite finished, no film formed at the surface, except 

 a ring formation along the niveau of the solution used. 



The ascospores are almost invariably in pairs. Each spore 

 is round in shape, and ranges from 3 to 5 /^ in size. 



Dextrose, levulose, galactose, saccharose, maltose, rafiinose, 

 but not melibiose, inulin, lactose and a-methylglucoside are 

 fermentable by this yeast. 



1) In spile of repeated cultivation of Rhizopus oligosporus on several kinds of 

 media, the scantiness of the sporangium forQiation remains yet constant. 



